ArticlePloS one2013
Targeted mutagenesis in the malaria mosquito using TALE nucleases.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed, 94 citations in OpenAlex.
- Mosquito-based malaria control through genetic, microbial and immunological innovations.Nature reviews. Microbiology · 2026Review
- A genome-wide CRISPR screen in Anopheles mosquito cells identifies fitness and immune cell function-related genes.Nature communications · 2025Article
- Engineering gene drive docking sites in a haplolethal locus in Anopheles gambiae.Scientific reports · 2025Article
- CRISPR technologies for the control and study of malaria-transmitting anopheline mosquitoes.Parasites & vectors · 2025Review
- History of Mosquito Transgenesis: A Perspective in Review.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Eliminating malaria vectors with precision-guided sterile males.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- CRISPR-mediated germline mutagenesis for genetic sterilization of Anopheles gambiae males.Scientific reports · 2024Article
- Eliminating Malaria Vectors with Precision Guided Sterile Males.bioRxiv : the preprint server for biology · 2023Article
- A confinable female-lethal population suppression system in the malaria vector,Science advances · 2023Article
- Article
- Vectorial capacity and TEP1 genotypes of Anopheles gambiae sensu lato mosquitoes on the Kenyan coast.Parasites & vectors · 2022Article
- Driving down malaria transmission with engineered gene drives.Frontiers in genetics · 2022Review
- Article
- Vector biology meets disease control: using basic research to fight vector-borne diseases.Nature microbiology · 2019Review
- Molecular tools to create new strains for mosquito sexing and vector control.Parasites & vectors · 2018Review
- CRISPR-Cas9 Mediated Genome Editing inMethods and protocols · 2018Article
- Highly Efficient Site-Specific Mutagenesis in Malaria Mosquitoes Using CRISPR.G3 (Bethesda, Md.) · 2018Article
- Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector,Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Gene Drive for Mosquito Control: Where Did It Come from and Where Are We Headed?International journal of environmental research and public health · 2017Review
- Naming CRISPR alleles: endonuclease-mediated mutation nomenclature across species.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anopheles gambiae, the main mosquito vector of human malaria, is a challenging organism to manipulate genetically. As a consequence, reverse genetics studies in this disease vector have been largely limited to RNA interference experiments. Here, we report the targeted disruption of the immunity gene TEP1 using transgenic expression of Transcription-Activator Like Effector Nucleases (TALENs), and the isolation of several TEP1 mutant A. gambiae lines. These mutations inhibited protein production and rendered TEP1 mutants hypersusceptible to Plasmodium berghei. The TALEN technology opens up new avenues for genetic analysis in this disease vector and may offer novel biotechnology-based approaches for malaria control.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.