ArticlePloS one2013
Cell density- and quorum sensing-dependent expression of type VI secretion system 2 in Vibrio parahaemolyticus.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 44 citations in OpenAlex.
- Toxin Secretion Systems Used by Marine Pathogenic Bacteria: A Review.Journal of fish diseases · 2026Review
- The crosstalk between acute hepatopancreatic necrosis disease (AHPND) and the Vibrio quorum sensing (QS) system: A review.PLoS pathogens · 2026Review
- Functional divergence and regulatory network of the type VI secretion system inJournal of bacteriology · 2026Review
- The multifunctional type VI secretion system ofApplied and environmental microbiology · 2025Review
- AphA-dependent c-di-GMP production inJournal of bacteriology · 2025Article
- Quorum sensing regulates virulence factors in the coral pathogenApplied and environmental microbiology · 2025Article
- TssL2 of T6SS2 is required for mobility, biofilm formation, wrinkly phenotype formation, and virulence of Vibrio parahaemolyticus SH112.Applied microbiology and biotechnology · 2024Article
- Vibrio parahaemolyticus Down-Regulates the Intracellular c-di-GMP Level to Promote Swarming Motility by Sensing Surface.Current microbiology · 2024Article
- AcsS Negatively Regulates the Transcription of type VI Secretion System 2 Genes in Vibrio parahaemolyticus.Current microbiology · 2024Article
- A bacterial binary toxin system that kills both insects and aquatic crustaceans: Photorhabdus insect-related toxins A and B.PLoS pathogens · 2023Review
- Quorum sensing and QsvR tightly control the transcription ofFrontiers in microbiology · 2023Article
- QsvR and OpaR coordinately repress biofilm formation byFrontiers in microbiology · 2023Article
- Dynamics of the protein search for targets on DNA in quorum-sensing cells.Biophysical journal · 2022Article
- Expression of the AHPND Toxins PirAInternational journal of molecular sciences · 2022Article
- Quorum Sensing Controls the CRISPR and Type VI Secretion Systems inFrontiers in veterinary science · 2022Article
- The quorum-sensing systems of Vibrio campbellii DS40M4 and BB120 are genetically and functionally distinct.Environmental microbiology · 2021Article
- Transcriptional regulation of the virulence genes and the biofilm formation associated operons in Vibrio parahaemolyticus.Gut pathogens · 2021Article
- A Review of the Functional Annotations of Important Genes in the AHPND-Causing pVA1 Plasmid.Microorganisms · 2020Review
- Quorum-Sensing Regulator OpaR Directly Represses Seven Protease Genes inFrontiers in microbiology · 2020Article
- Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology.Journal of microbiology (Seoul, Korea) · 2019Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVibrio parahaemolyticus AphA and OpaR are the two master quorum sensing (QS) regulators that are abundantly expressed at low cell density (LCD) and high cell density (HCD), respectively, with a feature of reciprocally gradient production of them with transition between LCD and HCD. The type VI secretion system 2 (T6SS2) gene cluster can be assigned into three putative operons, namely VPA1027-1024, VPA1043-1028, and VPA1044-1046. T6SS2 contributes to adhesion of V. parahaemolyticus to host cells. METHODOLOGY/PRINCIPAL
findingsOpaR box-like sequences were found within the upstream promoter regions of all the above three operons, while none of AphA box-like elements could be identified for them. The subsequent primer extension, LacZ fusion, electrophoretic mobility shift, and DNase I footprinting assays disclosed that OpaR bound to the promoter regions of these three operons to stimulate their transcription, while AphA negatively regulated their transcription most likely through acting on OpaR. This regulation led to a gradient increase of T6SS2 transcription with transition from LCD to HCD. CONCLUSIONS/SIGNIFICANCE: V. parahaemolyticus OpaR and AphA positively and negatively regulate T6SS2 expression, respectively, leading to a gradient elevation of T6SS2 expression with transition from LCD to HCD. T6SS2 genes are thus assigned as the QS regulon members in V. parahaemolyticus.
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