ArticlePloS one2013
Matrix-assisted laser desorption ionization--time of flight mass spectrometry: an emerging tool for the rapid identification of mosquito vectors.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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56 citing papers in PubMed.
- Making MALDI-TOF MS for entomological parameters accessible: A practical guide for in-house library creation.PloS one · 2025Article
- MALDI-TOF MS Profiling and Its Contribution to Mosquito-Borne Diseases: A Systematic Review.Insects · 2024Review
- Potential of MALDI-TOF MS biotyping to detect deltamethrin resistance in the dengue vector Aedes aegypti.PloS one · 2024Article
- Rapid and non-destructive identification of Anopheles gambiae and Anopheles arabiensis mosquito species using Raman spectroscopy via machine learning classification models.Malaria journal · 2023Article
- Evolution of MALDI-TOF MS Profiles from Lice and Fleas Preserved in Alcohol over Time.Insects · 2023Article
- Wing Interferential Patterns (WIPs) and machine learning for the classification of some Aedes species of medical interest.Scientific reports · 2023Article
- Using MALDI-TOF MS to Identify Mosquitoes from Senegal and the Origin of Their Blood Meals.Insects · 2023Article
- Identification of NeotropicalTropical medicine and infectious disease · 2023Article
- Article
- Mosquitoes and Mosquito-Borne Diseases in Vietnam.Insects · 2022Review
- Applications of MALDI-TOF Mass Spectrometry to the Identification of Parasites and Arthropod Vectors of Human Diseases.Microorganisms · 2022Review
- MALDI-TOF MS Identification of Dromedary Camel Ticks and Detection of Associated Microorganisms, Southern Algeria.Microorganisms · 2022Article
- Mosquito Vectors (Diptera:Insects · 2022Review
- Enhanced procedures for mosquito identification by MALDI-TOF MS.Parasites & vectors · 2022Article
- MALDI-TOF mass spectrometry identification of mosquitoes collected in Vietnam.Parasites & vectors · 2022Article
- Article
- The Use of MALDI-TOF MS as a Diagnostic Tool for AdultFrontiers in veterinary science · 2022Article
- Wolbachia detection in Aedes aegypti using MALDI-TOF MS coupled to artificial intelligence.Scientific reports · 2021Article
- MALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes.PLoS neglected tropical diseases · 2021Article
- Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam.PLoS neglected tropical diseases · 2021Article
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Authors and funding
11 authors.
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Abstract
backgroundThe identification of mosquito vectors is typically based on morphological characteristics using morphological keys of determination, which requires entomological expertise and training. The use of protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), which is increasingly being used for the routine identification of bacteria, has recently emerged for arthropod identification.
methodsTo investigate the usefulness of MALDI-TOF-MS as a mosquito identification tool, we tested protein extracts made from mosquito legs to create a database of reference spectra. The database included a total of 129 laboratory-reared and field-caught mosquito specimens consisting of 20 species, including 4 Aedes spp., 9 Anopheles spp., 4 Culex spp., Lutzia tigripes, Orthopodomyia reunionensis and Mansonia uniformis. For the validation study, blind tests were performed with 76 specimens consisting of 1 to 4 individuals per species. A cluster analysis was carried out using the MALDI-Biotyper and some spectra from all mosquito species tested.
resultsBiomarker mass sets containing 22 and 43 masses have been detected from 100 specimens of the Anopheles, Aedes and Culex species. By carrying out 3 blind tests, we achieved the identification of mosquito vectors at the species level, including the differentiation of An. gambiae complex, which is possible using MALDI-TOF-MS with 1.8 as the cut-off identification score. A cluster analysis performed with all available mosquito species showed that MALDI-Biotyper can distinguish between specimens at the subspecies level, as demonstrated for An gambiae M and S, but this method cannot yet be considered a reliable tool for the phylogenetic study of mosquito species.
conclusionsWe confirmed that even without any specific expertise, MALDI-TOF-MS profiling of mosquito leg protein extracts can be used for the rapid identification of mosquito vectors. Therefore, MALDI-TOF-MS is an alternative, efficient and inexpensive tool that can accurately identify mosquitoes collected in the field during entomological surveys.
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