ArticlePloS one2013
Raman microspectroscopy analysis in the treatment of acanthamoeba keratitis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Genotypic and Phenotypic Characterisation ofPathogens (Basel, Switzerland) · 2026Article
- A Pilot Study on Single-Cell Raman Spectroscopy Combined with Machine Learning for Phenotypic Characterization ofMicroorganisms · 2025Article
- Discrimination between Carbapenem-Resistant and Carbapenem-Sensitive Klebsiella pneumoniae Strains through Computational Analysis of Surface-Enhanced Raman Spectra: a Pilot Study.Microbiology spectrum · 2022Article
- A simple and reliable approach for the fabrication of nanoporous silver patterns for surface-enhanced Raman spectroscopy applications.Scientific reports · 2021Article
- Article
- Scalable nanolaminated SERS multiwell cell culture assay.Microsystems & nanoengineering · 2020Article
- Experimental study of the mechanisms leading to the formation of glistenings in intraocular lenses by Raman spectroscopy.Biomedical optics express · 2019Article
- Time resolved and label free monitoring of extracellular metabolites by surface enhanced Raman spectroscopy.PloS one · 2017Article
- The antimicrobial polymer PHMB enters cells and selectively condenses bacterial chromosomes.Scientific reports · 2016Article
- Autophagy inhibitors as a potential antiamoebic treatment for Acanthamoeba keratitis.Antimicrobial agents and chemotherapy · 2015Article
- Evaluation of the activity of new cationic carbosilane dendrimers on trophozoites and cysts of Acanthamoeba polyphaga.Parasitology research · 2015Article
- Pathogen- and Host-Directed Antileishmanial Effects Mediated by Polyhexanide (PHMB).PLoS neglected tropical diseases · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acanthamoeba keratitis is a rare but serious corneal disease, often observed in contact lens wearers. Clinical treatment of infected patients frequently involves the use of polyhexamethylene biguanide (PHMB), a polymer used as a disinfectant and antiseptic, which is toxic also for the epithelial cells of the cornea. Prompt and effective diagnostic tools are hence highly desiderable for both starting early therapy and timely suspension of the treatment. In this work we use Raman microspectroscopy to analyse in vitro a single Acanthamoeba cell in cystic phase. In particular, we investigate the effect of PHMB at the single-cell level, providing useful information on both the underlying biochemical mechanism and the time frame for Acanthamoeba eradication in ocular infections. Furthermore, we demonstrate that Raman spectroscopy, in conjunction with standard multivariate analysis methods, allows discriminating between live and dead Acanthamoebas, which is fundamental to optimizing patients' treatment.
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