ArticlePloS one2013
Impact of Hfq on global gene expression and intracellular survival in Brucella melitensis.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 57 citations in OpenAlex.
- Contribution of Hfq to gene regulation and virulence inInfection and immunity · 2024Article
- The RNA chaperone Hfq has a multifaceted role inFrontiers in cellular and infection microbiology · 2024Article
- MicroRNA-155 expression with Brucella infection in vitro and in vivo and decreased serum levels of MicroRNA-155 in patients with brucellosis.Scientific reports · 2022Article
- Brucella melitensis invA gene (BME_RS01060) transcription is promoted under acidic stress conditions.Archives of microbiology · 2021Article
- Uncovering the Hidden Credentials ofMicrobiology and molecular biology reviews : MMBR · 2021Review
- Comparative Genome-Wide Transcriptome Analysis ofFrontiers in microbiology · 2021Article
- Proteomics of Brucella.Proteomes · 2020Review
- Regulation of mRNA Stability During Bacterial Stress Responses.Frontiers in microbiology · 2020Review
- Rewiring the functional complexity between Crc, Hfq and sRNAs to regulate carbon catabolite repression in Pseudomonas.World journal of microbiology & biotechnology · 2019Review
- Analysis of protein expression inJournal of veterinary science · 2018Article
- Transcriptional regulator GntR of Brucella abortus regulates cytotoxicity, induces the secretion of inflammatory cytokines and affects expression of the type IV secretion system and quorum sensing system in macrophages.World journal of microbiology & biotechnology · 2017Article
- Analyzing the Complex Regulatory Landscape of Hfq - an Integrative, Multi-Omics Approach.Frontiers in microbiology · 2017Article
- The Glutaminase-Dependent System Confers Extreme Acid Resistance to New Species and Atypical Strains ofFrontiers in microbiology · 2017Article
- The RNA-Binding Chaperone Hfq Is an Important Global Regulator of Gene Expression in Pasteurella multocida and Plays a Crucial Role in Production of a Number of Virulence Factors, Including Hyaluronic Acid Capsule.Infection and immunity · 2016Article
- Large-scale identification of small noncoding RNA with strand-specific deep sequencing and characterization of a novel virulence-related sRNA in Brucella melitensis.Scientific reports · 2016Article
- Transcriptome-Wide Identification of Hfq-Associated RNAs in Brucella suis by Deep Sequencing.Journal of bacteriology · 2016Article
- Establishment of Chronic Infection: Brucella's Stealth Strategy.Frontiers in cellular and infection microbiology · 2016Review
- RNA-seq reveals the critical role of OtpR in regulating Brucella melitensis metabolism and virulence under acidic stress.Scientific reports · 2015Article
- Deletion of the Small RNA Chaperone Protein Hfq down Regulates Genes Related to Virulence and Confers Protection against Wild-Type Brucella Challenge in Mice.Frontiers in microbiology · 2015Article
- Identification of a Novel Small Non-Coding RNA Modulating the Intracellular Survival of Brucella melitensis.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
18 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brucella melitensis is a facultative intracellular bacterium that replicates within macrophages. The ability of brucellae to survive and multiply in the hostile environment of host macrophages is essential to its virulence. The RNA-binding protein Hfq is a global regulator that is involved in stress resistance and pathogenicity. Here we demonstrate that Hfq is essential for stress adaptation and intracellular survival in B. melitensis. A B. melitensis hfq deletion mutant exhibits reduced survival under environmental stresses and is attenuated in cultured macrophages and mice. Microarray-based transcriptome analyses revealed that 359 genes involved in numerous cellular processes were dysregulated in the hfq mutant. From these same samples the proteins were also prepared for proteomic analysis to directly identify Hfq-regulated proteins. Fifty-five proteins with significantly affected expression were identified in the hfq mutant. Our results demonstrate that Hfq regulates many genes and/or proteins involved in metabolism, virulence, and stress responses, including those potentially involved in the adaptation of Brucella to the oxidative, acid, heat stress, and antibacterial peptides encountered within the host. The dysregulation of such genes and/or proteins could contribute to the attenuated hfq mutant phenotype. These findings highlight the involvement of Hfq as a key regulator of Brucella gene expression and facilitate our understanding of the role of Hfq in environmental stress adaptation and intracellular survival of B. melitensis.
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