ArticlePloS one2013
HPat a decapping activator interacting with the miRNA effector complex.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- Cap-binding protein 4EHP effects translation silencing by microRNAs.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- GW-Bodies and P-Bodies Constitute Two Separate Pools of Sequestered Non-Translating RNAs.PloS one · 2016Article
- General and MicroRNA-Mediated mRNA Degradation Occurs on Ribosome Complexes in Drosophila Cells.Molecular and cellular biology · 2015Article
- Analysis of RNA Interference Lines Identifies New Functions of Maternally-Expressed Genes Involved in Embryonic Patterning in Drosophila melanogaster.G3 (Bethesda, Md.) · 2015Article
- Roles of mRNA fate modulators Dhh1 and Pat1 in TNRC6-dependent gene silencing recapitulated in yeast.The Journal of biological chemistry · 2015Article
- The mRNA decay factor PAT1 functions in a pathway including MAP kinase 4 and immune receptor SUMM2.The EMBO journal · 2015Article
- The complexity of miRNA-mediated repression.Cell death and differentiation · 2015Review
- Novel roles of the multi-functional CCR4-NOT complex in post-transcriptional regulation.Frontiers in genetics · 2014Review
- The role of disordered protein regions in the assembly of decapping complexes and RNP granules.Genes & development · 2013Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Animal miRNAs commonly mediate mRNA degradation and/or translational repression by binding to their target mRNAs. Key factors for miRNA-mediated mRNA degradation are the components of the miRNA effector complex (AGO1 and GW182) and the general mRNA degradation machinery (deadenylation and decapping enzymes). The CCR4-NOT1 complex required for the deadenylation of target mRNAs is directly recruited to the miRNA effector complex. However, it is unclear whether the following decapping step is only a consequence of deadenylation occurring independent of the miRNA effector complex or e.g. decapping activators can get recruited to the miRNA effector complex. In this study we performed split-affinity purifications in Drosophila cells and provide evidence for the interaction of the decapping activator HPat with the miRNA effector complex. Furthermore, in knockdown analysis of various mRNA degradation factors we demonstrate the importance of NOT1 for this interaction. This suggests that deadenylation and/or the recruitment of NOT1 protein precedes the association of HPat with the miRNA effector complex. Since HPat couples deadenylation and decapping, the recruitment of HPat to the miRNA effector complex provides a mechanism to commit the mRNA target for degradation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.