ArticlePloS one2013
Boron nitride nanotube-mediated stimulation of cell co-culture on micro-engineered hydrogels.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 102 citations in OpenAlex.
- Micropatterned Styrene-Butadiene-Styrene Thin Films Doped with Barium Titanate Nanoparticles: Effects on Myoblast Differentiation.ACS biomaterials science & engineering · 2025Article
- Nanotechnology-Fortified Manipulation of Cell CaSmall science · 2024Article
- Ultrasound Stimulation of Piezoelectric Nanocomposite Hydrogels Boosts Chondrogenic DifferentiationACS nano · 2024Article
- Stimulation strategies for electrical and magnetic modulation of cells and tissues.Cell regeneration (London, England) · 2023Review
- Piezoelectric Nanomaterials Activated by Ultrasound in Disease Treatment.Pharmaceutics · 2023Review
- In Vitro and In Vivo Cytotoxicity of Boron Nitride Nanotubes: A Systematic Review.Nanomaterials (Basel, Switzerland) · 2022Review
- Bioactive Chitosan-Based Organometallic Scaffolds for Tissue Engineering and Regeneration.Topics in current chemistry (Cham) · 2022Review
- Graphene Oxide and Reduced Graphene Oxide Nanoflakes Coated with Glycol Chitosan, Propylene Glycol Alginate, and Polydopamine: Characterization and Cytotoxicity in Human Chondrocytes.Nanomaterials (Basel, Switzerland) · 2021Article
- Ultrasound-Induced Wireless Energy Harvesting: From Materials Strategies to Functional Applications.Nano energy · 2020Article
- Biomaterials in Tendon and Skeletal Muscle Tissue Engineering: Current Trends and Challenges.Materials (Basel, Switzerland) · 2018Review
- Ultrasound-mediated piezoelectric differentiation of neuron-like PC12 cells on PVDF membranes.Scientific reports · 2017Article
- Nanostructured ultra-thin patches for ultrasound-modulated delivery of anti-restenotic drug.International journal of nanomedicine · 2016Article
- Synthesis of boron nitride nanotubes and their applications.Beilstein journal of nanotechnology · 2015Review
- Skeletal muscle tissue engineering: methods to form skeletal myotubes and their applications.Tissue engineering. Part B, Reviews · 2014Review
Corrections and comments
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Authors and funding
12 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper, we describe the effects of the combination of topographical, mechanical, chemical and intracellular electrical stimuli on a co-culture of fibroblasts and skeletal muscle cells. The co-culture was anisotropically grown onto an engineered micro-grooved (10 µm-wide grooves) polyacrylamide substrate, showing a precisely tuned Young's modulus (∼ 14 kPa) and a small thickness (∼ 12 µm). We enhanced the co-culture properties through intracellular stimulation produced by piezoelectric nanostructures (i.e., boron nitride nanotubes) activated by ultrasounds, thus exploiting the ability of boron nitride nanotubes to convert outer mechanical waves (such as ultrasounds) in intracellular electrical stimuli, by exploiting the direct piezoelectric effect. We demonstrated that nanotubes were internalized by muscle cells and localized in both early and late endosomes, while they were not internalized by the underneath fibroblast layer. Muscle cell differentiation benefited from the synergic combination of topographical, mechanical, chemical and nanoparticle-based stimuli, showing good myotube development and alignment towards a preferential direction, as well as high expression of genes encoding key proteins for muscle contraction (i.e., actin and myosin). We also clarified the possible role of fibroblasts in this process, highlighting their response to the above mentioned physical stimuli in terms of gene expression and cytokine production. Finally, calcium imaging-based experiments demonstrated a higher functionality of the stimulated co-cultures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.