ArticlePloS one2013
High-throughput analysis of stimulus-evoked behaviors in Drosophila larva reveals multiple modality-specific escape strategies.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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Who cites it
66 citing papers in PubMed.
- Region-specific mechanosensation modulateseLife · 2026Article
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- Visual Threat Location Impacts Brain-Wide Visual Adaptation Networks.The Journal of comparative neurology · 2026Article
- A positive feedback loop between sensory and octopaminergic neurons underlies nociceptive plasticity in Drosophila larvae.PLoS genetics · 2026Article
- Ascending nociceptive pathways drive rapid escape and sustained avoidance in adultbioRxiv : the preprint server for biology · 2025Article
- Article
- Feeding state-dependent neuropeptidergic modulation of reciprocally interconnected inhibitory neurons biases sensorimotor decisions in Drosophila.Nature communications · 2025Article
- Neural substrates of cold nociception ineLife · 2025Article
- Statistical signature of subtle behavioral changes in large-scale assays.PLoS computational biology · 2025Article
- Neural circuits underlying context-dependent competition between defensive actions in Drosophila larvae.Nature communications · 2025Article
- Mechanically evoked spike responses of pentascolopidial chordotonal organs of Drosophila melanogaster larvae.The Journal of experimental biology · 2024Article
- Feedback inhibition by a descending GABAergic neuron regulates timing of escape behavior ineLife · 2024Article
- LarvaTagger: manual and automatic tagging of Drosophila larval behaviour.Bioinformatics (Oxford, England) · 2024Article
- Article
- Article
- The connectome of an insect brain.Science (New York, N.Y.) · 2023Article
- Continuous, long-term crawling behavior characterized by a robotic transport system.bioRxiv : the preprint server for biology · 2023Article
- Temperature sensitivity and temperature response across development in theFrontiers in molecular neuroscience · 2023Article
- Linking neural circuits to the mechanics of animal behavior inFrontiers in neural circuits · 2023Review
- Nociception in fruit fly larvae.Frontiers in pain research (Lausanne, Switzerland) · 2023Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
All organisms react to noxious and mechanical stimuli but we still lack a complete understanding of cellular and molecular mechanisms by which somatosensory information is transformed into appropriate motor outputs. The small number of neurons and excellent genetic tools make Drosophila larva an especially tractable model system in which to address this problem. We developed high throughput assays with which we can simultaneously expose more than 1,000 larvae per man-hour to precisely timed noxious heat, vibration, air current, or optogenetic stimuli. Using this hardware in combination with custom software we characterized larval reactions to somatosensory stimuli in far greater detail than possible previously. Each stimulus evoked a distinctive escape strategy that consisted of multiple actions. The escape strategy was context-dependent. Using our system we confirmed that the nociceptive class IV multidendritic neurons were involved in the reactions to noxious heat. Chordotonal (ch) neurons were necessary for normal modulation of head casting, crawling and hunching, in response to mechanical stimuli. Consistent with this we observed increases in calcium transients in response to vibration in ch neurons. Optogenetic activation of ch neurons was sufficient to evoke head casting and crawling. These studies significantly increase our understanding of the functional roles of larval ch neurons. More generally, our system and the detailed description of wild type reactions to somatosensory stimuli provide a basis for systematic identification of neurons and genes underlying these behaviors.
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