ArticlePloS one2013
A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
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Who cites it
100 citing papers in PubMed, 208 citations in OpenAlex.
- Simultaneous Detection of Human Norovirus GI, GII and Hepatitis A Virus Using CRISPR-Cas12a-Based RT-RPA and Lateral Flow Strip Method.Food and environmental virology · 2026Article
- A Novel Diagnostic Tool for West Nile Virus Lineage 1a and 2 Using a CRISPR-Cas12a System.Biosensors · 2025Article
- Rapid detection of Seneca Valley virus based on reverse transcription recombinase polymerase amplification and CRISPR-Cas13a.Virulence · 2025Article
- Isothermal nucleic acid amplification assays for the detection of porcine stool-associated RNA virus.Scientific reports · 2025Article
- Rapid and specific on-site H5Nx avian influenza diagnosis via RPA and PAM-independent CRISPR-Cas12a assay combined with anti-NP antibody-based viral RNA purification.Frontiers in veterinary science · 2025Article
- Advances in Virus Detection Techniques Based on Recombinant Polymerase Amplification.Molecules (Basel, Switzerland) · 2024Review
- Rapid and Direct Detection of the Stubby Root Nematode,International journal of molecular sciences · 2024Article
- Advances in plant pathogen detection: integrating recombinase polymerase amplification with CRISPR/Cas systems.3 Biotech · 2024Review
- Review
- Detection of foot-and-mouth disease virus RNA using a closed loop-mediated isothermal amplification system.Frontiers in microbiology · 2024Article
- Establishment and application of a rapid molecular diagnostic platform for the isothermal visual amplification of group B Streptococcus based on recombinase polymerase.Frontiers in cellular and infection microbiology · 2024Article
- A rapid and visual detection assay for Senecavirus A based on recombinase-aided amplification and lateral flow dipstick.Frontiers in cellular and infection microbiology · 2024Article
- Rapid detection of alveolar echinococcosis in hepatic nodules of horses by recombinase polymerase amplification assay.Veterinary and animal science · 2023Article
- Rapid molecular detection of CMY-2, and CTX-M group 1 and 9 variants via recombinase polymerase amplification.JAC-antimicrobial resistance · 2023Article
- A novel reverse transcription recombinase polymerase amplification assay for rapid detection of GI.1 genotype of rabbit hemorrhagic disease virus.Frontiers in veterinary science · 2023Article
- Research Progress on the detection methods of porcine reproductive and respiratory syndrome virus.Frontiers in microbiology · 2023Review
- A Combined Method Based on the FIPV N Monoclonal Antibody Immunofluorescence Assay and RT-nPCR Method for the Rapid Diagnosis of FIP-Suspected Ascites.Transboundary and emerging diseases · 2023Article
- Development and Visualization Improvement for the Rapid Detection of Decapod Iridescent Virus 1 (DIV1) inViruses · 2022Article
- Article
- Rapid detection of human coronavirus NL63 by isothermal reverse transcription recombinase polymerase amplification.Journal of clinical virology plus · 2022Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Foot-and-mouth disease (FMD) is a trans-boundary viral disease of livestock, which causes huge economic losses and constitutes a serious infectious threat for livestock farming worldwide. Early diagnosis of FMD helps to diminish its impact by adequate outbreak management. In this study, we describe the development of a real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of FMD virus (FMDV). The FMDV RT-RPA design targeted the 3D gene of FMDV and a 260 nt molecular RNA standard was used for assay validation. The RT-RPA assay was fast (4-10 minutes) and the analytical sensitivity was determined at 1436 RNA molecules detected by probit regression analysis. The FMDV RT-RPA assay detected RNA prepared from all seven FMDV serotypes but did not detect classical swine fever virus or swine vesicular disease virus. The FMDV RT-RPA assay was used in the field during the recent FMD outbreak in Egypt. In clinical samples, reverse transcription polymerase chain reaction (RT-PCR) and RT-RPA showed a diagnostic sensitivity of 100% and 98%, respectively. In conclusion, FMDV RT-RPA was quicker and much easier to handle in the field than real-time RT-PCR. Thus RT-RPA could be easily implemented to perform diagnostics at quarantine stations or farms for rapid spot-of-infection detection.
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