ArticlePloS one2013
Notch pathway activation contributes to inhibition of C2C12 myoblast differentiation by ethanol.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Alcohol Consumption During Muscle Disuse Causes Differential Signaling Responses in a Muscle-Specific Manner in Mice.International journal of molecular sciences · 2026Article
- Cystine/glutamate antiporter xCT controls skeletal muscle glutathione redox, bioenergetics and differentiation.Redox biology · 2024Article
- Alcohol and Skeletal Muscle in Health and Disease.Alcohol research : current reviews · 2023Review
- NLK is required for Ras/ERK/SRF/ELK signaling to tune skeletal muscle development by phosphorylating SRF and antagonizing the SRF/MKL pathway.Cell death discovery · 2022Article
- A Pillar-Based High-Throughput Myogenic Differentiation Assay to Assess Drug Safety.Molecules (Basel, Switzerland) · 2021Article
- Article
- Chronic Alcohol Dysregulates Skeletal Muscle Myogenic Gene Expression after Hind Limb Immobilization in Female Rats.Biomolecules · 2020Article
- Article
- Loss of tafazzin results in decreased myoblast differentiation in C2C12 cells: A myoblast model of Barth syndrome and cardiolipin deficiency.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2018Article
- Methylmercury exposure causes a persistent inhibition of myogenin expression and C2C12 myoblast differentiation.Toxicology · 2018Article
- Autocrine signaling is a key regulatory element during osteoclastogenesis.Biology open · 2014Article
- Chronic binge alcohol consumption alters myogenic gene expression and reduces in vitro myogenic differentiation potential of myoblasts from rhesus macaques.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The loss of muscle mass in alcoholic myopathy may reflect alcohol inhibition of myogenic cell differentiation into myotubes. Here, using a high content imaging system we show that ethanol inhibits C2C12 myoblast differentiation by reducing myogenic fusion, creating smaller and less complex myotubes compared with controls. Ethanol administration during C2C12 differentiation reduced MyoD and myogenin expression, and microarray analysis identified ethanol activation of the Notch signaling pathway target genes Hes1 and Hey1. A reporter plasmid regulated by the Hes1 proximal promoter was activated by alcohol treatment in C2C12 cells. Treatment of differentiating C2C12 cells with a gamma secretase inhibitor (GSI) abrogated induction of Hes1. On a morphological level GSI treatment completely rescued myogenic fusion defects and partially restored other myotube parameters in response to alcohol. We conclude that alcohol inhibits C2C12 myoblast differentiation and the inhibition of myogenic fusion is mediated by Notch pathway activation.
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