ArticleJournal of materials chemistry2012
Combinatorial screening of chemically defined human mesenchymal stem cell culture substrates.
Article in Journal of materials chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 29 citations in OpenAlex.
- Phenotypic technologies in stem cell biology.Cell chemical biology · 2021Review
- Synthetic alternatives to Matrigel.Nature reviews. Materials · 2020Article
- Controlled Self-assembly of Stem Cell Aggregates Instructs Pluripotency and Lineage Bias.Scientific reports · 2017Article
- Development of peptide-functionalized synthetic hydrogel microarrays for stem cell and tissue engineering applications.Acta biomaterialia · 2016Article
- Surface functionalization and dynamics of polymeric cell culture substrates.Current opinion in biotechnology · 2016Review
- Human iPSC-derived endothelial cell sprouting assay in synthetic hydrogel arrays.Acta biomaterialia · 2016Article
- Polymer microarray technology for stem cell engineering.Acta biomaterialia · 2016Review
- Polyethylene Glycol Coatings on Plastic Substrates for Chemically Defined Stem Cell Culture.Advanced healthcare materials · 2015Article
- Biomaterial arrays with defined adhesion ligand densities and matrix stiffness identify distinct phenotypes for tumorigenic and nontumorigenic human mesenchymal cell types.Biomaterials science · 2014Article
- Context clues: the importance of stem cell-material interactions.ACS chemical biology · 2014Review
- Patterned self-assembled monolayers: efficient, chemically defined tools for cell biology.Chembiochem : a European journal of chemical biology · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Self-assembled monolayers (SAMs) of alkanethiolates on gold are chemically defined substrates that can be used to evaluate the effects of an immobilized biomolecule. However, the types of biomolecules that can influence stem cell behavior are numerous and inter-related, and efficient experimental formats are a critical need. Here we employed a SAM array technology to investigate the effects of multiple, distinct peptides and peptide combinations on human mesenchymal stem cell (hMSC) behavior. Specifically, we characterized the conjugation of peptide mixtures to SAM arrays and then investigated the combined effects of a bone morphogenic protein receptor-binding peptide (BR-BP), a heparin proteoglycan-binding peptide (HPG-BP), and varied densities of the integrin-binding ligand Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP) on hMSC surface coverage and alkaline phosphatase activity. Results indicate that an amine reactive fluorescent probe can be used to characterize peptide composition after immobilization in SAM array spots. Furthermore, hMSC response to BR-BP and HPG-BP is dependent on GRGDSP density and at day 7, hMSC alkaline phosphatase expression is highly dependent on GRGDSP density. Taken together, we demonstrate how a SAM array approach can be used to probe the combinatorial effects of multiple peptides and motivate further investigations into potential synergies between cell adhesion and other bioactive peptides.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.