ArticleThe Plant cell2013
The clathrin adaptor complex AP-2 mediates endocytosis of brassinosteroid insensitive1 in Arabidopsis.
Article in The Plant cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.
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Who cites it
113 citing papers in PubMed, 193 citations in OpenAlex.
- The role of clathrin in post-Golgi secretion in plant cells.The New phytologist · 2026Article
- Clathrin-Mediated Endocytosis in Plants: Historical to Modern Advances.Traffic (Copenhagen, Denmark) · 2026Review
- Ligand-induced ubiquitination regulates endocytosis and homeostasis of the ERECTA receptor kinase for stomatal development.The New phytologist · 2026Article
- Natural Variations of ZmRLR1 Mediate the Root Lodging Resistance of Maize by Regulating Root Ascorbate and Auxin Homeostasis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Cargo Sorting Zones in Plant Trans-Golgi Network.Sub-cellular biochemistry · 2026Review
- Dynamic ASK1 proximity networks uncover SCF-dependent and noncanonical roles in ABA and drought adaptation.bioRxiv : the preprint server for biology · 2025Article
- Stage-specific transcriptomic responses of wheat to heat, drought, and combined stress: AP2/ERF regulatory networks and functional characterization of TaEREBP1-L.BMC plant biology · 2025Article
- A combined biochemical and computational approach provides evidence for membrane remodelling by the structural scaffold of the endocytic TPLATE complex.Nature plants · 2025Article
- De novo transcriptome assembly and gene expression analysis of Cnidium officinale under high-temperature conditions.BMC genomics · 2025Article
- Canonical tyrosine-based motifs are required for constitutive endocytosis and polarity of IRT1 and contribute to metal uptake.The Plant journal : for cell and molecular biology · 2025Article
- Identification of an adaptor protein 2σ gene for OsTGW12 to determine grain weight and potentiate quality breeding in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Brassinosteroid Signaling Dynamics: Ubiquitination-Dependent Regulation of Core Signaling Components.International journal of molecular sciences · 2025Review
- Genome-wide identification of the adaptor protein complexes and its expression patterns analysis in foxtail millet (Setaria italica L.).BMC plant biology · 2025Article
- Immunoisolation of Plant Endosomal Vesicles to Explore Uptake of Pathogen Effector Proteins During Infection of Nicotiana benthamiana.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Repeated global adaptation across plant species.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- FERONIA adjusts CC1 phosphorylation to control microtubule array behavior in response to salt stress.Science advances · 2024Article
- Role of the dynamin-related protein 2 family and SH3P2 in clathrin-mediated endocytosis in Arabidopsis thaliana.Journal of cell science · 2024Article
- The cell surface is the place to be for brassinosteroid perception and responses.Nature plants · 2024Review
- Unraveling the molecular landscape of lead-induced cochlear synaptopathy: a quantitative proteomics analysis.Frontiers in cellular neuroscience · 2024Article
- TPLATE complex-dependent endocytosis attenuates CLAVATA1 signaling for shoot apical meristem maintenance.EMBO reports · 2023Article
53 more citing papers are in PubMed but not listed here.
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Authors and funding
17 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clathrin-mediated endocytosis (CME) regulates many aspects of plant development, including hormone signaling and responses to environmental stresses. Despite the importance of this process, the machinery that regulates CME in plants is largely unknown. In mammals, the heterotetrameric adaptor protein complex-2 (AP-2) is required for the formation of clathrin-coated vesicles at the plasma membrane (PM). Although the existence of AP-2 has been predicted in Arabidopsis thaliana, the biochemistry and functionality of the complex is still uncharacterized. Here, we identified all the subunits of the Arabidopsis AP-2 by tandem affinity purification and found that one of the large AP-2 subunits, AP2A1, localized at the PM and interacted with clathrin. Furthermore, endocytosis of the leucine-rich repeat receptor kinase, brassinosteroid insensitive1 (BRI1), was shown to depend on AP-2. Knockdown of the two Arabidopsis AP2A genes or overexpression of a dominant-negative version of the medium AP-2 subunit, AP2M, impaired BRI1 endocytosis and enhanced the brassinosteroid signaling. Our data reveal that the CME machinery in Arabidopsis is evolutionarily conserved and that AP-2 functions in receptor-mediated endocytosis.
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