ArticleMethods in molecular biology (Clifton, N.J.)2013
Compartmentalized neuronal cultures.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Article
- Fetal Brain Injury Models of Fetal Alcohol Syndrome: Examination of Neuronal Morphologic Condition Using Sholl Assay.Methods in molecular biology (Clifton, N.J.) · 2021Article
- HIV-1 and HIV-1-Tat Induce Mitochondrial DNA Damage in Human Neurons.Journal of HIV and AIDS · 2020Article
- TNF-α/TNFR2 Regulatory Axis Stimulates EphB2-Mediated Neuroregeneration Via Activation of NF-κB.Journal of cellular physiology · 2016Article
- Visualizing directional Rab7 and TrkA cotrafficking in axons by pTIRF microscopy.Methods in molecular biology (Clifton, N.J.) · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The culturing of neurons results in formation of the layer of neurons with random extensive overlapping outgrowth. To understand specific roles of somas, axons, and dendrites in complex function of neurons and to identify molecular mechanisms of biological processes in these cellular compartments various methods were developed. We utilized AXon Investigation System (AXIS™) manufactured by Millipore. This device provides an opportunity to orient neuronal outgrowth and spatially isolate neuronal processes from neuronal bodies. AXIS device is a slide-mounted microfluidic system, which consists of four wells. Two of the wells are connected by a channel on each side of the device. Channels are connected by microgrooves (approximately 120). The size of microgrooves (10 μm in width and 5 μm in height) do not permit passage of cell through while allowing extension of neurites. The microfluidic design also allows an establishment of a hydrostatic gradient when the volume in one chamber is greater than that in the other (Park et al. Nat Protocols 1: 2128-2136, 2006). This feature allows studying of the effect of specific compounds on selected compartments of neurons.
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Registered trials
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