ArticleMethods in molecular biology (Clifton, N.J.)2013
Isolation and propagation of primary human and rodent embryonic neural progenitor cells and cortical neurons.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Neurons in a Dish: A Review of In Vitro Cell Models for Studying Neurogenesis.Journal of neurochemistry · 2026Review
- Prenatal Alcohol Exposure Inhibits Transient Expression of Autophagy and Synaptic Proteins in Developing Brain.Obstetrics and gynecology research · 2025Article
- Neuroprotective Role of DING protein in Fetal Alcohol Spectrum Disorders and Depression.Obstetrics and gynecology research · 2025Article
- Exosomal Lipid Biomarkers of Oligodendrocyte Pathology to Predict Scoliosis in Children with Cerebral Palsy.Obstetrics and gynecology research · 2023Article
- Heterocellular spheroids of the neurovascular blood-brain barrier as a platform for personalized nanoneuromedicine.iScience · 2021Article
- Fetal Brain Injury Models of Fetal Alcohol Syndrome: Examination of Neuronal Morphologic Condition Using Sholl Assay.Methods in molecular biology (Clifton, N.J.) · 2021Article
- HIV-1 and HIV-1-Tat Induce Mitochondrial DNA Damage in Human Neurons.Journal of HIV and AIDS · 2020Article
- Differentiation of Mesenchymal Stem Cells to Neuroglia: in the Context of Cell Signalling.Stem cell reviews and reports · 2019Review
- Novel Peptidomic Approach for Identification of Low and High Molecular Weight Tauopathy Peptides Following Calpain Digestion, and Primary Culture Neurotoxic Challenges.International journal of molecular sciences · 2019Article
- Glia-neuron interactions in neurological diseases: Testing non-cell autonomy in a dish.Brain research · 2017Review
- TNF-α/TNFR2 Regulatory Axis Stimulates EphB2-Mediated Neuroregeneration Via Activation of NF-κB.Journal of cellular physiology · 2016Article
- HIV-1 Tat and Cocaine Impair Survival of Cultured Primary Neuronal Cells via a Mitochondrial Pathway.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2016Article
- Expression of Signaling Molecules in Progressive Multifocal Leukoencephalopathy.Current HIV research · 2016Article
- Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research.Molecular brain · 2014Article
- Modeling neurodevelopmental disorders using human pluripotent stem cells.Stem cell reviews and reports · 2014Review
- Involvement of IRS-1 interaction with ADAM10 in the regulation of neurite extension.Journal of cellular physiology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The research on human neural progenitor cells holds great potential for the understanding the molecular programs that control differentiation of cells of glial and neuronal lineages and pathogenetic mechanisms of neurological diseases. Stem cell technologies provide also opportunities for pharmaceutical industry to develop new approaches for regenerative medicine. Here we describe the protocol for isolation and maintenance of neural progenitor cells and cortical neurons using human fetal brain tissue. This protocol can be successfully adapted for preparation of rodent neural and oligodendrocyte progenitor cells. While several methods for isolation of neural and oligodendrocyte progenitors from rodent brain tissue have been described, including techniques which use gene transfer and magnetic resonance beads, few methods are focused on derivation of human oligodendrocyte progenitor cells. Development of human culture provides the most physiologically relevant system for investigation of mechanisms which regulate function of oligodendrocyte, specifically of human origin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.