Evidence map›Paper›PMID 23975776›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2013

Membrane protein structure determination: back to the membrane.

Yong Yao, Yi Ding, Ye Tian, Stanley J Opella, Francesca M Marassi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
3.5field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Yong YaoSanford Burnham Medical Research Institute, La Jolla, CA, USA.
Yi Ding
Ye Tian
Stanley J Opella
Francesca M Marassi
Sanford Burnham Prebys Medical Discovery Institute · USUniversity of California, San Diego · US

Funding

Tumor Microenvironment and Cancer ImmunologyP30CA030199 · NCI · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI Michael Jackson · 1985 to 2026
$107.2M
RESOURCE FOR SOLID STATE NMR OF PROTEINSP41EB002031 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2003 to 2018
$16.3M
Molecular Imaging of G-Protein-Coupled Receptors for Drug DevelopmentR01EB005161 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2006 to 2014
$6.8M
Host recognition by pathogenic yersiniaeR01GM100265 · NIGMS · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI MARASSI, FRANCESCA M · 2012 to 2015
$1.7M
Structure Determination of Membrane Proteins in Phospholipid BilyaersR01GM099986 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI OPELLA, STANLEY J · 2012 to 2015
$1.1M
Computational methods for NMR structure determination of proteins in membranesR21GM094727 · NIGMS · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI MARASSI, FRANCESCA M · 2010 to 2011
$509k
Structure of- beta barrel membrane protein-lipid bi(RMI)R21GM075917 · NIGMS · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI MARASSI, FRANCESCA M · 2005 to 2006
$474k
NCI NIH HHS P30 CA030199NIBIB NIH HHS P41 EB002031NIBIB NIH HHS R01 EB005161NIGMS NIH HHS R01 GM099986NIGMS NIH HHS R01 GM100265NIGMS NIH HHS R21 GM075917NIGMS NIH HHS R21 GM094727
6 · The paper itself

Abstract

NMR spectroscopy enables the structures of membrane proteins to be determined in the native-like environment of the phospholipid bilayer membrane. This chapter outlines the methods for membrane protein structural studies using solid-state NMR spectroscopy with samples of membrane proteins incorporated in proteoliposomes or planar lipid bilayers. The methods for protein expression and purification, sample preparation, and NMR experiments are described and illustrated with examples from OmpX and Ail, two bacterial outer membrane proteins that function in bacterial virulence.

Indexed as

Models, MolecularBacterial Outer Membrane ProteinsCell MembraneCrystallography, X-RayEscherichia coli ProteinsHydrolasesLipid BilayersNuclear Magnetic Resonance, BiomolecularProtein ConformationYersinia pestisBacterial Outer Membrane ProteinsEscherichia coli ProteinsHydrolasesLipid BilayersOmpX protein, E coli

Identifiers

PMID23975776
PMCPMC4012560
OpenAlexW82123799

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.