ArticleNature protocols2013
Plasmonic ELISA for the detection of analytes at ultralow concentrations with the naked eye.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed.
- Review
- Advancements and Emerging Trends in the Detection of Sweet Potato Diseases: A Comprehensive Review.ACS omega · 2026Review
- Nanomaterial-Enhanced Biosensing: Mechanisms and Emerging Applications.Advanced healthcare materials · 2025Review
- Development of a Gold Nanoparticle-Based ELISA for Detection of PCV2.Pathogens (Basel, Switzerland) · 2024Article
- Plasmonic Nanostructure Biosensors: A Review.Sensors (Basel, Switzerland) · 2023Review
- Gold Nanoparticle-Based Plasmonic Biosensors.Biosensors · 2023Review
- Allosteric aptasensor-initiated target cycling and transcription amplification of light-up RNA aptamer for sensitive detection of protein.Sensors and actuators. B, Chemical · 2022Article
- The sequestration mechanism as a generalizable approach to improve the sensitivity of biosensors and bioassays.Chemical science · 2022Article
- An Immunocolorimetric Sensing System for Highly Sensitive and High-Throughput Detection of BNP with Carbon-Gold Nanocomposites Amplification.Biosensors · 2022Article
- Molecular Fingerprinting of the Omicron Variant Genome of SARS-CoV-2 by SERS Spectroscopy.Nanomaterials (Basel, Switzerland) · 2022Article
- Application of microfluidic chips in anticancer drug screening.Bosnian journal of basic medical sciences · 2022Review
- Paper-based genetic assays with bioconjugated gold nanorods and an automated readout pipeline.Scientific reports · 2022Article
- Noble Metal Nanoparticle Biosensors: From Fundamental Studies toward Point-of-Care Diagnostics.Accounts of chemical research · 2022Review
- A colorimetric method for determination of the prostate specific antigen based on enzyme-free cascaded signal amplification via peptide-copper(II) nanoparticles.Mikrochimica acta · 2020Article
- Nanomaterials-Based Colorimetric Immunoassays.Nanomaterials (Basel, Switzerland) · 2019Review
- Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.Chemical reviews · 2019Review
- A Metal Chelator as a Plasmonic Signal-Generation Superregulator for Ultrasensitive Colorimetric Bioassays of Disease Biomarkers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Enzyme-catalyzed Ag Growth on Au Nanoparticle-assembled Structure for Highly Sensitive Colorimetric Immunoassay.Scientific reports · 2018Article
- Plasmonic molecular assays: Recent advances and applications for mobile health.Nano research · 2018Review
- Nanomaterial-based biosensors for detection of prostate specific antigen.Mikrochimica acta · 2017Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This protocol describes a signal-generation mechanism for the naked-eye detection of analytes at low concentrations with ELISA. The key step is to generate solutions of desired tonality by growing gold nanoparticles with a particular state of aggregation. This is accomplished by linking the growth of gold nanoparticles with the biocatalytic cycle of the enzyme label. The protocol adapts a conventional ELISA procedure with catalase-labeled antibodies. The enzyme consumes hydrogen peroxide, and then gold (III) ions are added to generate gold nanoparticles. The concentration of hydrogen peroxide dictates the state of aggregation of gold nanoparticles. This allows for the naked-eye detection of analytes by observing the generation of blue- or red-colored gold nanoparticle solutions. When coupled with conventional ELISA, this signal-generation procedure allows for the naked-eye detection of analytes within 1 h.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.