ArticleNucleic acids research2013
Elucidating the evolutionary conserved DNA-binding specificities of WRKY transcription factors by molecular dynamics and in vitro binding assays.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers.
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98 citing papers in PubMed.
- Functional and regulatory complexity: challenges and prospects for understanding rice WRKY transcription factors.Journal of experimental botany · 2026Review
- How WRKY transcription factors fine-tune specificity in plant stress responses: from W-box to regulatory code.Molecular biology reports · 2026Review
- Melatonin-inducedFrontiers in plant science · 2026Article
- The specificity landscape of WRKY transcription factors reveals the bidirectional influence of non-CG methylation.Nucleic acids research · 2025Article
- The transcription factor WRKY25 can act as redox switch to drive the expression of WRKY53 during leaf senescence in arabidopsis.Scientific reports · 2025Article
- Genome-Wide Characterization ofInternational journal of molecular sciences · 2025Article
- DNA-Protein Binding is Dominated by Short Anchoring Elements.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Full-length transcriptome profiling ofPeerJ · 2025Article
- Identification, characterization, and expression analysis of WRKY transcription factors in Cardamine violifolia reveal the key genes involved in regulating selenium accumulation.BMC plant biology · 2024Article
- Genome-Wide Identification of theInternational journal of molecular sciences · 2024Article
- Differential heat-response characteristics of two plastid isoforms of triose phosphate isomerase in tomato.Plant biotechnology journal · 2024Article
- Genome-wide identification, stress- and hormone-responsive expression characteristics, and regulatory pattern analysis of Scutellaria baicalensis SbSPLs.Plant molecular biology · 2024Article
- Genome-wide analysis of WRKY gene family and the dynamic responses of key WRKY genes involved in cadmium stress inFrontiers in plant science · 2024Article
- WRKY Transcription Factors inBiomolecules · 2023Article
- WRKY transcription factors and OBERON histone-binding proteins form complexes to balance plant growth and stress tolerance.The EMBO journal · 2023Article
- KaScape: a sequencing-based method for global characterization of protein‒DNA binding affinity.Scientific reports · 2023Article
- Genome-Wide Identification of Bilberry WRKY Transcription Factors: Go Wild and Duplicate.Plants (Basel, Switzerland) · 2023Article
- Exploring the Binding Affinity of the ARR2 GARP DNA Binding Domain via Comparative Methods.Genes · 2023Article
- Rice WRKY13 TF protein binds to motifs in the promoter region to regulate downstream disease resistance-related genes.Functional & integrative genomics · 2023Article
- Transcriptome-Wide Identification of the GRAS Transcription Factor Family inInternational journal of molecular sciences · 2023Article
38 more citing papers are in PubMed but not listed here.
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5 authors.
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Abstract
WRKY transcription factors constitute a large protein family in plants that is involved in the regulation of developmental processes and responses to biotic or abiotic stimuli. The question arises how stimulus-specific responses are mediated given that the highly conserved WRKY DNA-binding domain (DBD) exclusively recognizes the 'TTGACY' W-box consensus. We speculated that the W-box consensus might be more degenerate and yet undetected differences in the W-box consensus of WRKYs of different evolutionary descent exist. The phylogenetic analysis of WRKY DBDs suggests that they evolved from an ancestral group IIc-like WRKY early in the eukaryote lineage. A direct descent of group IIc WRKYs supports a monophyletic origin of all other group II and III WRKYs from group I by loss of an N-terminal DBD. Group I WRKYs are of paraphyletic descent and evolved multiple times independently. By homology modeling, molecular dynamics simulations and in vitro DNA-protein interaction-enzyme-linked immunosorbent assay with AtWRKY50 (IIc), AtWRKY33 (I) and AtWRKY11 (IId) DBDs, we revealed differences in DNA-binding specificities. Our data imply that other components are essentially required besides the W-box-specific binding to DNA to facilitate a stimulus-specific WRKY function.
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