ArticleArchives of virology2014
Failure of propagation of human norovirus in intestinal epithelial cells with microvilli grown in three-dimensional cultures.
Article in Archives of virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 64 citations in OpenAlex.
- Article
- In Vitro Culture of Human Norovirus in the Last 20 Years.Biomedicines · 2024Review
- Development of an intestinal mucosaJournal of virology · 2024Article
- Review
- Current trends and new approaches for human norovirus replication in cell culture: a literature review.Archives of virology · 2024Review
- Replication of Human Norovirus in Human Intestinal Enteroids Is Affected by Fecal Sample Processing.Viruses · 2024Article
- 3D engineered tissue models for studying human-specific infectious viral diseases.Bioactive materials · 2023Review
- A Roadmap for Building Waterborne Virus Traps.JACS Au · 2022Review
- From the Dish to the Real World: Modeling Interactions between the Gut and Microorganisms in Gut Organoids by Tailoring the Gut Milieu.International journal of stem cells · 2022Review
- Inactivation of Murine Norovirus Suspended in Organic Matter Simulating Actual Conditions of Viral Contamination.Food and environmental virology · 2021Article
- Bile Goes Viral.Viruses · 2021Review
- Review
- Targeted Profiling of Immunological Genes during Norovirus Replication in Human Intestinal Enteroids.Viruses · 2021Article
- Roles of bile acids in enteric virus replication.Animal diseases · 2021Review
- Review
- Human Norovirus Replication in Temperature-Optimized MDCK Cells by Forkhead Box O1 Inhibition.Journal of microbiology and biotechnology · 2020Article
- Human Norovirus Cultivation in Nontransformed Stem Cell-Derived Human Intestinal Enteroid Cultures: Success and Challenges.Viruses · 2019Review
- A semi-nested RT-PCR assay for detection of norovirus in rat fecal samples.Experimental animals · 2019Article
- Human Norovirus Replication in Human Intestinal Enteroids as Model to Evaluate Virus Inactivation.Emerging infectious diseases · 2018Article
- Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Human noroviruses (HuNoVs) are a leading cause of acute gastroenteritis. Establishment of a cell culture system for in vitro HuNoV growth remains challenging. Replication of HuNoVs in human intestinal cell lines (INT-407 and Caco-2) that differentiate to produce microvilli in rotation wall vessel (RWV) three-dimensional cultures has been reported (Straub et al. in Emerg Infect Dis 13:396-403, 2007; J Water Health 9:225-240, 2011, and Water Sci Technol 67:863-868, 2013). We used a similar RWV system, intestinal cell lines, and the same (Genogroup [G] I.1) plus additional (GII.4 and GII.12) HuNoV strains to test the system's reproducibility and to expand the earlier findings. Apical microvilli were observed on the surface of both cell lines by light and electron microscopy. However, none of the cell types tested resulted in productive viral replication of any of the HuNoV strains, as confirmed by plateau or declining viral RNA titers in the supernatants and cell lysates of HuNoV-infected cells, determined by real-time reverse transcription PCR. These trends were the same when culture supplements were added that have been reported to be effective for replication of other fastidious enteric viruses in vitro. Additionally, by confocal microscopy and orthoslice analysis, viral capsid proteins were mainly observed above the actin filament signals, which suggested that the majority of viral antigens were on the cell surface. We conclude that even intestinal cells displaying microvilli were not sufficient to support HuNoV replication under the conditions tested.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.