ArticleImmunogenetics2013
Sequencing of modern Lepus VDJ genes shows that the usage of VHn genes has been retained in both Oryctolagus and Lepus that diverged 12 million years ago.
Article in Immunogenetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- The evolutionary history of IGKC in mammals reveals ancient duplications and remarkable divergence in lagomorphs.Frontiers in immunology · 2025Article
- The IgA of hares (Frontiers in immunology · 2023Article
- Evolutionary analyses of polymeric immunoglobulin receptor (pIgR) in the mammals reveals an outstanding mutation rate in the lagomorphs.Frontiers in immunology · 2022Article
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- Computational Strategies for Dissecting the High-Dimensional Complexity of Adaptive Immune Repertoires.Frontiers in immunology · 2018Review
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- Evolutionary study of leporid CD4 reveals a hotspot of genetic variability within the D2 domain.Immunogenetics · 2016Article
- An overview of the lagomorph immune system and its genetic diversity.Immunogenetics · 2016Review
- Genetic characterization of interleukins (IL-1α, IL-1β, IL-2, IL-4, IL-8, IL-10, IL-12A, IL-12B, IL-15 and IL-18) with relevant biological roles in lagomorphs.Innate immunity · 2015Article
- Genetic diversity comparison of the DQA gene in European rabbit (Oryctolagus cuniculus) populations.Immunogenetics · 2015Article
- Field and experimental data indicate that the eastern cottontail (Sylvilagus floridanus) is susceptible to infection with European brown hare syndrome (EBHS) virus and not with rabbit haemorrhagic disease (RHD) virus.Veterinary research · 2015Article
- Sequencing of Sylvilagus VDJ genes reveals a new VHa allelic lineage and shows that ancient VH lineages were retained differently in leporids.Immunogenetics · 2014Article
- Leporid immunoglobulin G shows evidence of strong selective pressure on the hinge and CH3 domains.Open biology · 2014Article
- Evolution of viral sensing RIG-I-like receptor genes in Leporidae genera Oryctolagus, Sylvilagus, and Lepus.Immunogenetics · 2014Article
- Article
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6 authors.
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Abstract
Among mammals, the European rabbit (Oryctolagus cuniculus) has a unique mechanism of generating the primary antibody repertoire. Despite having over 200 VH genes, the VH1 gene, the most D-proximal VH gene, is used in 80-90 % of VDJ rearrangements, while the remaining 10-20 % is encoded by the VHn genes that map at least 100 Kb upstream of VH1. The maintenance of the VHn genes usage in low frequency in VDJ rearrangements has been suggested to represent a relic of an ancestral immunologic response to pathogens. To address this question, we sequenced VDJ genes for another leporid, genus Lepus, which separated from European rabbit 12 million years ago. Approximately 25 VDJ gene sequences were obtained for each one of three Lepus europaeus individuals. We found that Lepus also uses the VHn genes in 5-10 % of its VDJ rearrangements. Our results show that the VHn genes are a conserved ancestral polymorphism that has been maintained in the leporids genome and is being used for the generation of VDJ rearrangements by both modern Lepus and Oryctolagus.
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