ArticleCellular and molecular life sciences : CMLS2014
CAS directly interacts with vinculin to control mechanosensing and focal adhesion dynamics.
Article in Cellular and molecular life sciences : CMLS, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 61 citations in OpenAlex.
- The mechanical response of vinculin.Science advances · 2025Article
- Review
- Article
- TRPV4 activation enhances compressive properties and glycosaminoglycan deposition of equine neocartilage sheets.Osteoarthritis and cartilage open · 2022Article
- Maintaining proteostasis under mechanical stress.EMBO reports · 2021Review
- Article
- Manipulation of Focal Adhesion Signaling by Pathogenic Microbes.International journal of molecular sciences · 2021Review
- p130Cas/Frontiers in cell and developmental biology · 2021Review
- Context-Dependent Role of Vinculin in Neutrophil Adhesion, Motility and Trafficking.Scientific reports · 2020Article
- Article
- The interaction of p130Cas with PKN3 promotes malignant growth.Molecular oncology · 2019Article
- Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells.Nature communications · 2018Article
- Structural and functional insights into the interaction between the Cas family scaffolding protein p130Cas and the focal adhesion-associated protein paxillin.The Journal of biological chemistry · 2017Article
- Structural characterization of CAS SH3 domain selectivity and regulation reveals new CAS interaction partners.Scientific reports · 2017Article
- Vinculin in cell-cell and cell-matrix adhesions.Cellular and molecular life sciences : CMLS · 2017Review
- Molecular stretching modulates mechanosensing pathways.Protein science : a publication of the Protein Society · 2017Review
- The role of focal adhesion anchoring domains of CAS in mechanotransduction.Scientific reports · 2017Article
- MicroRNA-203 mimics age-related aortic smooth muscle dysfunction of cytoskeletal pathways.Journal of cellular and molecular medicine · 2017Article
- Review
- Identification of Novel Crk-associated Substrate (p130Cas) Variants with Functionally Distinct Focal Adhesion Kinase Binding Activities.The Journal of biological chemistry · 2015Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Focal adhesions are cellular structures through which both mechanical forces and regulatory signals are transmitted. Two focal adhesion-associated proteins, Crk-associated substrate (CAS) and vinculin, were both independently shown to be crucial for the ability of cells to transmit mechanical forces and to regulate cytoskeletal tension. Here, we identify a novel, direct binding interaction between CAS and vinculin. This interaction is mediated by the CAS SRC homology 3 domain and a proline-rich sequence in the hinge region of vinculin. We show that CAS localization in focal adhesions is partially dependent on vinculin, and that CAS-vinculin coupling is required for stretch-induced activation of CAS at the Y410 phosphorylation site. Moreover, CAS-vinculin binding significantly affects the dynamics of CAS and vinculin within focal adhesions as well as the size of focal adhesions. Finally, disruption of CAS binding to vinculin reduces cell stiffness and traction force generation. Taken together, these findings strongly implicate a crucial role of CAS-vinculin interaction in mechanosensing and focal adhesion dynamics.
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