ArticleChimerism
Detection and quantification of chimerism by droplet digital PCR.
Article in Chimerism. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Chimerism analysis by ABO blood group genotyping with digital droplet PCR.International journal of hematology · 2025Article
- Can novel methods replace the gold standard chimerism method after allogeneic hematopoietic stem cell transplantation?Annals of hematology · 2024Review
- Viral airway injury promotes cell engraftment in an in vitro model of cystic fibrosis cell therapy.American journal of physiology. Lung cellular and molecular physiology · 2024Article
- Expression of gain-of-function CFTR in cystic fibrosis airway cells restores epithelial function better than wild-type or codon-optimized CFTR.Molecular therapy. Methods & clinical development · 2023Article
- New methods for the quantification of mixed chimerism in transplantation.Frontiers in immunology · 2023Article
- Validation of next-generation sequencing-based chimerism testing for accurate detection and monitoring of engraftment in hematopoietic stem cell transplantation.Frontiers in genetics · 2023Article
- Chimerism analysis for clinicians: a review of the literature and worldwide practices.Bone marrow transplantation · 2022Review
- A practical guide to chimerism analysis: Review of the literature and testing practices worldwide.Human immunology · 2021Review
- Important factors in implementation of lineage-specific chimerism analysis for routine use.Bone marrow transplantation · 2021Article
- Comparison of single nucleotide polymorphisms and short tandem repeats as markers for differentiating between donors and recipients in solid organ transplantation.Korean journal of transplantation · 2021Article
- Fetal microchimerism and implications for maternal health.Obstetric medicine · 2020Review
- Digital PCR: A Reliable Tool for Analyzing and Monitoring Hematologic Malignancies.International journal of molecular sciences · 2020Review
- Development of a Digital RT-PCR Method for Absolute Quantification of Bluetongue Virus in Field Samples.Frontiers in veterinary science · 2020Article
- Current Trends in Applications of Circulatory Microchimerism Detection in Transplantation.International journal of molecular sciences · 2019Review
- Digital PCR in Myeloid Malignancies: Ready to Replace Quantitative PCR?International journal of molecular sciences · 2019Review
- An ultrasensitive test for profiling circulating tumor DNA using integrated comprehensive droplet digital detection.Lab on a chip · 2019Article
- Detection of Human Microchimerism following Allogeneic Cell Transplantation Using Droplet Digital PCR.Stem cells international · 2019Article
- Systematic comparison of donor chimerism in peripheral blood and bone marrow after hematopoietic stem cell transplantation.Blood cancer journal · 2017Article
- A Child's HLA-DRB1 genotype increases maternal risk of systemic lupus erythematosus.Journal of autoimmunity · 2016Article
- Digital PCR Panel for Sensitive Hematopoietic Chimerism Quantification after Allogeneic Stem Cell Transplantation.International journal of molecular sciences · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate quantification of chimerism and microchimerism is proving to be increasingly valuable for hematopoietic cell transplantation as well as non-transplant conditions. However, methods that are available to quantify low-level chimerism lack accuracy. Therefore, we developed and validated a method for quantifying chimerism based on digital PCR technology. We demonstrate accurate quantification that far exceeds what is possible with analog qPCR down to 0.01% with the potential to go even lower. Also, this method is inherently more informative than qPCR. We expect the advantages of digital PCR will make it the preferred method for chimerism analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.