ArticleMicrobiology (Reading, England)2013
The β-sliding clamp directs the localization of HdaA to the replisome in Caulobacter crescentus.
Article in Microbiology (Reading, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Review
- Computational modeling of unphosphorylated CtrA:iScience · 2021Article
- Spatial coupling between DNA replication and mismatch repair in Caulobacter crescentus.Nucleic acids research · 2021Article
- HdaB: a novel and conserved DnaA-related protein that targets the RIDA process to stimulate replication initiation.Nucleic acids research · 2020Article
- Article
- Multilayered control of chromosome replication inBiochemical Society transactions · 2019Review
- Where and When Bacterial Chromosome Replication Starts: A Single Cell Perspective.Frontiers in microbiology · 2018Review
- Insufficient levels of the nrdAB-encoded ribonucleotide reductase underlie the severe growth defect of the Δhda E. coli strain.Molecular microbiology · 2017Article
- Replication fork passage drives asymmetric dynamics of a critical nucleoid-associated protein in Caulobacter.The EMBO journal · 2017Article
- Caulobacter crescentus intrinsic dimorphism provides a prompt bimodal response to copper stress.Nature microbiology · 2016Article
- The Caulobacter crescentus Homolog of DnaA (HdaA) Also Regulates the Proteolysis of the Replication Initiator Protein DnaA.Journal of bacteriology · 2015Article
- (p)ppGpp modulates cell size and the initiation of DNA replication in Caulobacter crescentus in response to a block in lipid biosynthesis.Microbiology (Reading, England) · 2015Article
- Redefining bacterial origins of replication as centralized information processors.Frontiers in microbiology · 2015Review
- Fifty years after the replicon hypothesis: cell-specific master regulators as new players in chromosome replication control.Journal of bacteriology · 2014Review
- How do bacteria localize proteins to the cell pole?Journal of cell science · 2014Review
- Preventing bacterial suicide: a novel toxin-antitoxin strategy.Molecular cell · 2013Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The initiation of chromosome replication is tightly regulated in bacteria to ensure that it takes place only once per cell cycle. In many proteobacteria, this process requires the ATP-bound form of the DnaA protein. The regulatory inactivation of DnaA (RIDA) facilitates the conversion of DnaA-ATP into replication-inactive DnaA-ADP, thereby preventing overinitiation. Homologues of the HdaA protein, together with the β-clamp of the DNA polymerase (DnaN), are required for this process. Here, we used fluorescence resonance energy transfer experiments to demonstrate that HdaA interacts with DnaN in live Caulobacter crescentus cells. We show that a QFKLPL motif in the N-terminal region of HdaA is required for this interaction and that this motif is also needed to recruit HdaA to the subcellular location occupied by the replisome during DNA replication. An HdaA mutant protein that cannot colocalize or interact with DnaN can also not support the essential function of HdaA. These results suggest that the recruitment of HdaA to the replisome is needed during RIDA in C. crescentus, probably as a means to sense whether chromosome replication has initiated before DnaA becomes inactivated. In addition, we show that a conserved R145 residue located in the AAA+ domain of HdaA is also needed for the function of HdaA, although it does not affect the interaction of HdaA with DnaN in vivo. The AAA+ domain of HdaA may therefore be required during RIDA after the initial recruitment of HdaA to the replisome by DnaN.
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