ArticleJournal of bacteriology2013
Probing bacterial metabolism during infection using high-resolution transcriptomics.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- An anaerobic pathogen rewires host metabolism to fuel oxidative growth in the inflamed gut.Cell · 2026Article
- Transcriptional changes related to metabolic and iron acquisition strategies of avian pathogenicFrontiers in cellular and infection microbiology · 2026Article
- Molecular mechanisms of co-infections.EMBO reports · 2025Review
- Small regulatory RNAs of oral streptococci and periodontal bacteria.The Japanese dental science review · 2021Review
- Article
- Molybdenum Enzymes and How They Support Virulence in Pathogenic Bacteria.Frontiers in microbiology · 2020Review
- Large-scale identification of pathogen essential genes during coinfection with sympatric and allopatric microbes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Small RNA repertoires and their intraspecies variation in Aggregatibacter actinomycetemcomitans.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- Oral microbiome and health.AIMS microbiology · 2018Review
- Defining Genetic Fitness Determinants and Creating Genomic Resources for an Oral Pathogen.Applied and environmental microbiology · 2017Article
- Quorum sensing of Streptococcus mutans is activated by Aggregatibacter actinomycetemcomitans and by the periodontal microbiome.BMC genomics · 2017Article
- Microbial Community Composition Impacts Pathogen Iron Availability during Polymicrobial Infection.PLoS pathogens · 2016Article
- Article
- QseBC, a two-component bacterial adrenergic receptor and global regulator of virulence in Enterobacteriaceae and Pasteurellaceae.Molecular oral microbiology · 2016Review
- Article
- A Commensal Bacterium Promotes Virulence of an Opportunistic Pathogen via Cross-Respiration.mBio · 2016Article
- Aggregatibacter actinomycetemcomitans QseBC is activated by catecholamines and iron and regulates genes encoding proteins associated with anaerobic respiration and metabolism.Molecular oral microbiology · 2015Article
- Metatranscriptomics reveals metabolic adaptation and induction of virulence factors by Haemophilus parasuis during lung infection.Veterinary research · 2015Article
- The cell envelope proteome of Aggregatibacter actinomycetemcomitans.Molecular oral microbiology · 2015Article
- From ingestion to colonization: the influence of the host environment on regulation of the LEE encoded type III secretion system in enterohaemorrhagic Escherichia coli.Frontiers in microbiology · 2015Review
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4 authors.
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Abstract
A fundamental aspect of most infectious diseases is the need for the invading microbe to proliferate in the host. However, little is known about the metabolic pathways required for pathogenic microbes to colonize and persist in their hosts. In this study, we used RNA sequencing (RNA-seq) to generate a high-resolution transcriptome of the opportunistic pathogen Aggregatibacter actinomycetemcomitans in vivo. We identified 691 A. actinomycetemcomitans transcriptional start sites and 210 noncoding RNAs during growth in vivo and as a biofilm in vitro. Compared to in vitro biofilm growth on a defined medium, ∼14% of the A. actinomycetemcomitans genes were differentially regulated in vivo. A disproportionate number of genes coding for proteins involved in metabolic pathways were differentially regulated in vivo, suggesting that A. actinomycetemcomitans in vivo metabolism is distinct from in vitro growth. Mutational analyses of differentially regulated genes revealed that formate dehydrogenase H and fumarate reductase are important A. actinomycetemcomitans fitness determinants in vivo. These results not only provide a high-resolution genomic analysis of a bacterial pathogen during in vivo growth but also provide new insight into metabolic pathways required for A. actinomycetemcomitans in vivo fitness.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.