ArticleMethods in cell biology2013
Studying kinetochore-fiber ultrastructure using correlative light-electron microscopy.
Article in Methods in cell biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Article
- In vitro BioID: mapping the CENP-A microenvironment with high temporal and spatial resolution.Molecular biology of the cell · 2019Article
- Functional analysis after rapid degradation of condensins and 3D-EM reveals chromatin volume is uncoupled from chromosome architecture in mitosis.Journal of cell science · 2018Article
- Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis.Journal of cell science · 2017Article
- 3D-CLEM Reveals that a Major Portion of Mitotic Chromosomes Is Not Chromatin.Molecular cell · 2016Article
- Auxin/AID versus conventional knockouts: distinguishing the roles of CENP-T/W in mitotic kinetochore assembly and stability.Open biology · 2016Article
- Article
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Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Electron microscopy (EM) has dominated high-resolution cellular imaging for over 50 years, thanks to its ability to resolve on nanometer-scale intracellular structures such as the microtubules of the mitotic spindle. It is advantageous to view the cell of interest prior to processing the sample for EM. Correlative light-electron microscopy (CLEM) is a technique that allows one to visualize cells of interest by light microscopy (LM) before being transferred to EM for ultrastructural examination. Here, we describe how CLEM can be applied as an effective tool to study the spindle apparatus of mitotic cells. This approach allows transfected cells of interest, in desirable stages of mitosis, to be followed from LM to EM. CLEM has often been considered as a technically challenging and laborious technique. In this chapter, we provide step-by-step pictorial guides that allow successful CLEM to be achieved. In addition, we explain how it is possible to vary the sectioning plane, allowing spindles and microtubules to be analyzed from different angles, and the outputs that can be obtained from these methods when applied to the study of kinetochore fiber ultrastructure.
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Registered trials
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