ArticleBiophysical journal2013
Ultrasensitivity by molecular titration in spatially propagating enzymatic reactions.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Chemical waves in reaction-diffusion networks of small organic molecules.Chemical science · 2025Article
- Computing Arithmetic Functions Using Immobilised Enzymatic Reaction Networks.Angewandte Chemie (International ed. in English) · 2023Article
- Reaction-Diffusion Patterning of DNA-Based Artificial Cells.Journal of the American Chemical Society · 2022Article
- Reverse and forward engineering of protein pattern formation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- Free-standing supramolecular hydrogel objects by reaction-diffusion.Nature communications · 2017Article
- Grip on complexity in chemical reaction networks.Beilstein journal of organic chemistry · 2017Review
- The nanotechnology of life-inspired systems.Nature nanotechnology · 2016Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Delineating design principles of biological systems by reconstitution of purified components offers a platform to gauge the influence of critical physicochemical parameters on minimal biological systems of reduced complexity. Here we unravel the effect of strong reversible inhibitors on the spatiotemporal propagation of enzymatic reactions in a confined environment in vitro. We use micropatterned, enzyme-laden agarose gels which are stamped on polyacrylamide films containing immobilized substrates and reversible inhibitors. Quantitative fluorescence imaging combined with detailed numerical simulations of the reaction-diffusion process reveal that a shallow gradient of enzyme is converted into a steep product gradient by addition of strong inhibitors, consistent with a mathematical model of molecular titration. The results confirm that ultrasensitive and threshold effects at the molecular level can convert a graded input signal to a steep spatial response at macroscopic length scales.
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Registered trials
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