ArticleBiophysical journal2013
Optoporation and genetic manipulation of cells using femtosecond laser pulses.
Article in Biophysical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Microfluidic Electroporation: Recent Advances and Applications.Advances in experimental medicine and biology · 2026Review
- Acidic pH Condition Enhances Femtosecond Laser Photoinjection of Plasmid DNA into Intact Tobacco BY‑2.ACS omega · 2025Article
- Advances and future trends in real-time precision optical control of chemical processes in live cells.Npj imaging · 2025Review
- Monitoring optoporated process on mammalian cells by real-time measurement of membrane resealing time.Journal of biomedical optics · 2023Article
- Novel opto-fluidic drug delivery system for efficient cellular transfection.Journal of nanobiotechnology · 2023Article
- Graphene-based cardiac sensors and actuators.Frontiers in bioengineering and biotechnology · 2023Review
- Laser-based molecular delivery and its applications in plant science.Plant methods · 2022Review
- The cellular response to plasma membrane disruption for nanomaterial delivery.Nano convergence · 2022Review
- Microfluidic Based Physical Approaches towards Single-Cell Intracellular Delivery and Analysis.Micromachines · 2021Review
- Intracellular Delivery of mRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation.Nano-micro letters · 2020Article
- Femtosecond Plasmonic Laser Nanosurgery (fs-PLN) mediated by molecularly targeted gold nanospheres at ultra-low pulse fluences.Scientific reports · 2020Article
- Non-Viral in Vitro Gene Delivery: It is Now Time to Set the Bar!Pharmaceutics · 2020Review
- Enzyme-Assisted Photoinjection of Megadalton Molecules into Intact Plant Cells Using Femtosecond Laser Amplifier.Scientific reports · 2019Article
- Transient Photoinactivation of Cell Membrane Protein Activity without Genetic Modification by Molecular Hyperthermia.ACS nano · 2019Article
- Laser-assisted optoporation of cells and tissues - a mini-review.Biomedical optics express · 2019Article
- Current Trends of Microfluidic Single-Cell Technologies.International journal of molecular sciences · 2018Review
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Analysis of poration-induced changes in cells from laser-activated plasmonic substrates.Biomedical optics express · 2017Article
- Nanothermometry: From Microscopy to Thermal Treatments.Chemphyschem : a European journal of chemical physics and physical chemistry · 2016Review
- Optically-controlled platforms for transfection and single- and sub-cellular surgery.Biophysical reviews · 2015Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Femtosecond laser optoporation is a powerful technique to introduce membrane-impermeable molecules, such as DNA plasmids, into targeted cells in culture, yet only a narrow range of laser regimes have been explored. In addition, the dynamics of the laser-produced membrane pores and the effect of pore behavior on cell viability and transfection efficiency remain poorly elucidated. We studied optoporation in cultured cells using tightly focused femtosecond laser pulses in two irradiation regimes: millions of low-energy pulses and two higher-energy pulses. We quantified the pore radius and resealing time as a function of incident laser energy and determined cell viability and transfection efficiency for both irradiation regimes. These data showed that pore size was the governing factor in cell viability, independently of the laser irradiation regime. For viable cells, larger pores resealed more quickly than smaller pores, ruling out a passive resealing mechanism. Based on the pore size and resealing time, we predict that few DNA plasmids enter the cell via diffusion, suggesting an alternative mechanism for cell transfection. Indeed, we observed fluorescently labeled DNA plasmid adhering to the irradiated patch of the cell membrane, suggesting that plasmids may enter the cell by adhering to the membrane and then being translocated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.