ArticleBMC infectious diseases2013
Diagnosis and subtype analysis of Blastocystis sp. in 442 patients in a hospital setting in the Netherlands.
Article in BMC infectious diseases, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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54 citing papers in PubMed, 111 citations in OpenAlex.
- Transient colonization with Blastocystis spp. after transmission via fecal microbiota transplantation.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Trial
- Effects of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei supplementation on the single-cell fecal parasitome in children with celiac disease autoimmunity: a randomized, double-blind placebo-controlled clinical trial.Parasites & vectors · 2023Trial
- Occurrence and subtyping of the intestinal protozoanFood and waterborne parasitology · 2026Article
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- Epidemiology ofPathogens (Basel, Switzerland) · 2023Review
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- Frequency, subtypes distribution, and risk factors ofNew microbes and new infections · 2023Article
- An update on the distribution ofHeliyon · 2022Article
- Prevalence and Associated Factors ofTropical medicine and infectious disease · 2022Article
- Detection, Molecular Identification and Transmission of the Intestinal ProtozoaMicroorganisms · 2022Article
- Subtype distribution ofGastroenterology and hepatology from bed to bench · 2022Review
- Identification and Genetic Characterization ofInfection and drug resistance · 2022Article
- Prevalence of Blastocystis and its association with Firmicutes/Bacteroidetes ratio in clinically healthy and metabolically ill subjects.BMC microbiology · 2021Article
- Prevalence, subtypes and risk factors of Blastocystis spp. infection among pre- and perimenopausal women.BMC infectious diseases · 2021Article
- Prevalence of Intestinal Parasitic Infections among Children in Europe over the Last Five Years.Tropical medicine and infectious disease · 2021Review
- Parasitic infections in Swiss children: Are we overtesting?BMC gastroenterology · 2021Article
- Molecular characterization of Blastocystis subtypes isolated in the city of Uberaba, Minas Gerais State, Brazil.Revista da Sociedade Brasileira de Medicina Tropical · 2021Article
- Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBlastocystis sp. are among the most commonly observed intestinal parasites in routine clinical parasitology. Blastocystis in humans consists of at least 9 genetic subtypes. Different subtypes of Blastocystis may be associated with differences in pathogenicity and symptomatology.
methodsAdvanced microscopy on two samples and sequence-confirmed PCR on a third sample from the same individual were used for Blastocystis diagnosis and subtype analyses on routine clinical samples in a university hospital.
resultsWith a combined gold standard of sequence-confirmed PCR and positive advanced microscopy, 107 out of 442 (24.2%) patients were diagnosed with Blastocystis. infection, which is a high frequency of detection in comparison to previous reports from industrialized countries. The sensitivity of microscopy and sequence-confirmed PCR was 99.1% (106/107) and 96.3% (103/107), respectively.Among 103 typable samples, subtype 3 was most abundant (n = 43, 42%), followed by subtypes 1 and 2 (both n = 23, 22%), subtype 4 (n = 12, 12%), and single samples with subtypes 6 (1%) and subtype 7 (1%). The prevalence of Blastocystis infection was 38% in patients from the Department of Tropical Medicine and 18% in patients from other departments.
conclusionsA high prevalence of Blastocystis infection was found with both advanced microscopy and sequence-confirmed PCR in our patient population. Most cases were caused by subtypes ST1, ST2, ST3 and ST4. A significantly higher prevalence was found among patients with a history of recent travel to tropical countries.
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