ArticleProceedings of the National Academy of Sciences of the United States of America2013
Microscopy ambient ionization top-down mass spectrometry reveals developmental patterning.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Peptide and protein assays using customizable bio-affinity arrays combined with ambient ionization mass spectrometry.Chemical science · 2021Article
- Adipocytes Provide Fatty Acids to Acute Lymphoblastic Leukemia Cells.Frontiers in oncology · 2021Article
- Mass Spectrometry Analysis of Intact Proteins from Crude Samples.Analytical chemistry · 2020Article
- In situ mass spectrometry analysis of intact proteins and protein complexes from biological substrates.Biochemical Society transactions · 2020Review
- Electroporation and Mass Spectrometry: A New Paradigm for In Situ Analysis of Intact Proteins Direct from Living Yeast Colonies.Analytical chemistry · 2020Article
- Analytical Techniques in Neuroscience: Recent Advances in Imaging, Separation, and Electrochemical Methods.Analytical chemistry · 2017Article
- Clinical Application of Ambient Ionization Mass Spectrometry.Mass spectrometry (Tokyo, Japan) · 2017Article
- Mass Spectrometry Imaging Reveals Elevated Glomerular ATP/AMP in Diabetes/obesity and Identifies Sphingomyelin as a Possible Mediator.EBioMedicine · 2016Article
- Ambient Mass Spectrometry Imaging Using Direct Liquid Extraction Techniques.Analytical chemistry · 2016Review
- Mass spectrometry tools and workflows for revealing microbial chemistry.The Analyst · 2015Review
- Visualizing life with ambient mass spectrometry.Current opinion in biotechnology · 2015Review
- Article
- Mechanisms of synergy in polymicrobial infections.Journal of microbiology (Seoul, Korea) · 2014Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
There is immense cellular and molecular heterogeneity in biological systems. Here, we demonstrate the utility of integrating an inverted light microscope with an ambient ionization source, nanospray electrospray desorption ionization, attached to a high-resolution mass spectrometer to characterize the molecular composition of mouse spinal cords. We detected a broad range of molecules, including peptides and proteins, as well as metabolites such as lipids, sugars, and other small molecules, including S-adenosyl methionine and glutathione, through top-down MS. Top-down analysis revealed variation in the expression of Hb, including the transition from fetal to adult Hb and heterogeneity in Hb subunits consistent with the genetic diversity of the mouse models. Similarly, temporal changes to actin-sequestering proteins β-thymosins during development were observed. These results demonstrate that interfacing microscopy with ambient ionization provides the means to perform targeted in situ ambient top-down mass spectral analysis to study the pattern of proteins, lipids, and sugars in biologically heterogeneous samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.