Evidence map›Paper›PMID 23968720›Full record

ArticleFEBS letters2013

Attenuation of fibroblast growth factor signaling by poly-N-acetyllactosamine type glycans.

Kazuhiro Sugihara, Toshiaki K Shibata, Kayoko Takata, Takako Kimura, Naohiro Kanayama, Roy Williams, Shingo Hatakeyama, Tomoya O Akama, Chu-Wei Kuo, Kay-Hooi Khoo and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 3 countries.

Kazuhiro SugiharaDepartment of Gynecology and Obstetrics, Hamamatsu University School of Medicine, Hamamatsu City, Shizuoka, Japan. Electronic address: sugihara@hama-med.ac.jp.
Toshiaki K Shibata
Kayoko Takata
Takako Kimura
Naohiro Kanayama
Roy Williams
Shingo Hatakeyama
Tomoya O Akama
Chu-Wei Kuo
Kay-Hooi Khoo
Michiko N Fukuda
Hamamatsu University School of Medicine · JPSanford Burnham Prebys Medical Discovery Institute · USInstitute of Biological Chemistry, Academia Sinica · TWHirosaki University · JPKansai University · JP

Funding

Structural Basis of Immune Recognition of Glycosylated AntigensU54GM062116 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI SOBIESZCZUK, PETER · 2001 to 2010
$80.3M
THE A(1,3)FUCOSYLTRANSFERASE GENES AND SELECTIN LIGAND EXPRESSIONP01CA071932 · NCI · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI FUKUDA, MINORU · 1996 to 2012
$15.5M
Biosynthetic pathway of corneal keratan sulfateR01EY014620 · NEI · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI AKAMA, TOMOYA O · 2003 to 2010
$2.7M
Protein-Glycan Interaction Resource of the CFGR24GM098791 · NIGMS · EMORY UNIVERSITY · PI CUMMINGS, RICHARD D, PAULSON, JAMES C · 2011 to 2013
$2.6M
NCI NIH HHS CA33859NCI NIH HHS P01 CA071932NEI NIH HHS EY014620NEI NIH HHS R01 EY014620NIGMS NIH HHS GM098791NIGMS NIH HHS GM62116NIGMS NIH HHS R24 GM098791NIGMS NIH HHS U54 GM062116
6 · The paper itself

Abstract

Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glycans (polyLacs), enhanced motility. Mass spectrometry analysis revealed that sperm-associated polyLacs are heavily fucosylated, consistent with Lewis Y antigen. Immunohistochemistry of epididymis using an anti-Lewis Y antibody before and after EBG treatment suggested that polyLacs carrying the Lewis Y epitope are synthesized in epididymal epithelia and secreted to seminal fluid. EBG-treated sperm elevated cAMP levels and calcium influx, indicating activation of fibroblast growth factor signaling. Seminal fluid polyLacs bound to FGFs in vitro, and impaired FGF-mediated signaling in HEK293T cells.

Indexed as

Fibroblast Growth FactorsGlycoside HydrolasesHEK293 CellsHumansMalePolysaccharidesProtein BindingSignal TransductionSpermatozoaSperm MotilityFibroblast Growth FactorsGlycoside Hydrolaseskeratan-sulfate endo-1,4-beta-galactosidasepoly-N-acetyllactosaminePolysaccharidesGlycan arrayHeparinKeratan sulfateMALDI-MSTyrosine phosphorylation

Identifiers

PMID23968720
PMCPMC4029356
OpenAlexW2004623873

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.