ArticleACS synthetic biology2013
Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold.
Article in ACS synthetic biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
83 citing papers in PubMed.
- Inducible CRISPRi enables efficient and high-fidelity genome editing in Streptomyces.Nucleic acids research · 2026Article
- Engineering an IndustrialMicroorganisms · 2026Article
- Developing the protein quality control system inSynthetic and systems biotechnology · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Synthetic Biology Strategies for Activating Cryptic BGCs inACS omega · 2026Review
- Construction and Diversification of Natural Product Biosynthetic Gene Clusters at High Efficiency and Accuracy.ACS synthetic biology · 2025Article
- A precisely regulated Streptomyces expression system pTZYp facilitated by the strong promoter stnY and cmt operon.Biotechnology letters · 2025Article
- Targeted high-level production of chuangxinmycin and its halogenated derivatives with antitubercular activity.Microbial cell factories · 2025Article
- Highly efficient CRISPR-Cas9 base editing inApplied and environmental microbiology · 2025Article
- A plug-and-play system for polycyclic tetramate macrolactam production and functionalization.Microbial cell factories · 2025Article
- CRISPR-Cas9-driven genome editing inFrontiers in microbiology · 2025Article
- Engineered cytosine base editor enabling broad-scope and high-fidelity gene editing in Streptomyces.Nature communications · 2024Article
- Metabolic engineering of Escherichia coli for the production of 5-aminolevulinic acid based on combined metabolic pathway modification and reporter-guided mutant selection (RGMS).Biotechnology for biofuels and bioproducts · 2024Article
- Engineering Streptomyces sp. CPCC 204095 for the targeted high-level production of isatropolone A by elucidating its pathway-specific regulatory mechanism.Microbial cell factories · 2024Article
- Refactoring the architecture of a polyketide gene cluster enhances docosahexaenoic acid production in Yarrowia lipolytica through improved expression and genetic stability.Microbial cell factories · 2023Article
- Engineering BioBricks for Deoxysugar Biosynthesis and Generation of New Tetracenomycins.ACS omega · 2023Article
- Systematic identification of endogenous strong constitutive promoters from the diazotrophic rhizosphere bacterium Pseudomonas stutzeri DSM4166 to improve its nitrogenase activity.Microbial cell factories · 2023Article
- Improving spinosad production by tuning expressions of the forosamine methyltransferase and the forosaminyl transferase to reduce undesired less active byproducts in the heterologous host Streptomyces albus J1074.Microbial cell factories · 2023Article
- Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its Subsequent Fermentation for Optimized Production.Methods in molecular biology (Clifton, N.J.) · 2023Article
- CRISPR/Cas9-mediated genome editing in vancomycin-producing strainFrontiers in bioengineering and biotechnology · 2023Article
23 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Natural products (secondary metabolites) are a rich source of compounds with important biological activities. Eliciting pathway expression is always challenging but extremely important in natural product discovery because an individual pathway is tightly controlled through a unique regulation mechanism and hence often remains silent under the routine culturing conditions. To overcome the drawbacks of the traditional approaches that lack general applicability, we developed a simple synthetic biology approach that decouples pathway expression from complex native regulations. Briefly, the entire silent biosynthetic pathway is refactored using a plug-and-play scaffold and a set of heterologous promoters that are functional in a heterologous host under the target culturing condition. Using this strategy, we successfully awakened the silent spectinabilin pathway from Streptomyces orinoci. This strategy bypasses the traditional laborious processes to elicit pathway expression and represents a new platform for discovering novel natural products.
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