ArticleAnalytical chemistry2013
Simultaneous light-directed synthesis of mirror-image microarrays in a photochemical reaction cell with flare suppression.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Synthesis of highly complex phosphorothioate-modified oligonucleotides on microarrays.Scientific reports · 2026Article
- Accelerated, high-quality photolithographic synthesis of RNA microarrays in situ.Science advances · 2024Article
- Nonaqueous Oxidation in DNA Microarray Synthesis Improves the Oligonucleotide Quality and Preserves Surface Integrity on Gold and Indium Tin Oxide Substrates.Analytical chemistry · 2024Article
- Enzymatic Synthesis of High-Density RNA Microarrays.Current protocols · 2023Article
- Sequence-dependence of Cy3 and Cy5 dyes in 3' terminally-labeled single-stranded DNA.Scientific reports · 2022Article
- Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA.RSC advances · 2022Article
- Sequence Preference and Initiator Promiscuity forACS synthetic biology · 2021Article
- Chemical and photochemical error rates in light-directed synthesis of complex DNA libraries.Nucleic acids research · 2021Article
- Low cost DNA data storage using photolithographic synthesis and advanced information reconstruction and error correction.Nature communications · 2020Article
- Specificity and Efficiency of the Uracil DNA Glycosylase-Mediated Strand Cleavage Surveyed on Large Sequence Libraries.Scientific reports · 2019Article
- Large-Scale Photolithographic Synthesis of Chimeric DNA/RNA Hairpin Microarrays To Explore Sequence Specificity Landscapes of RNase HII Cleavage.Biochemistry · 2019Article
- Encrypted Oligonucleotide Arrays for Molecular Authentication.ACS combinatorial science · 2019Article
- A Characterization of the DNA Data Storage Channel.Scientific reports · 2019Article
- High-Density RNA Microarrays Synthesized In Situ by Photolithography.Angewandte Chemie (International ed. in English) · 2018Article
- High-Efficiency Reverse (5'→3') Synthesis of Complex DNA Microarrays.Scientific reports · 2018Article
- Impact of free NFood & function · 2018Article
- Mapping the affinity landscape of Thrombin-binding aptamers on 2΄F-ANA/DNA chimeric G-Quadruplex microarrays.Nucleic acids research · 2017Article
- High-Power 365 nm UV LED Mercury Arc Lamp Replacement for Photochemistry and Chemical Photolithography.ACS sustainable chemistry & engineering · 2017Article
- N(ϵ) -Carboxymethyllysine Increases the Expression of miR-103/143 and Enhances Lipid Accumulation in 3T3-L1 Cells.Journal of cellular biochemistry · 2016Article
- Express photolithographic DNA microarray synthesis with optimized chemistry and high-efficiency photolabile groups.Journal of nanobiotechnology · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The use of photolabile protecting groups is a versatile and well-established means of synthesizing high complexity microarrays of biopolymers, such as nucleic acids and peptides, for high-throughput analysis. The synthesis takes place in a photochemical reaction cell which positions the microarray substrate at the focus of the optical system delivering the light and which can be connected to a fluidics system which delivers appropriate reagents to the surface in synchrony with the light exposure. Here we describe a novel photochemical reaction cell which allows for the simultaneous synthesis of microarrays on two substrates. The reaction cell positions both substrates within the limited depth-of-focus of the optical system while maintaining the necessary reagent flow conditions. The resulting microarrays are mirror images of each other but otherwise essentially identical. The new reaction cell doubles the throughput of microarray synthesis without increasing the consumption of reagents. In addition, a secondary flow chamber behind the reaction cell can be filled with an absorbent and index-matching fluid to eliminate reflections from light exiting the reaction cell assembly, greatly reducing unintended light exposure that reduces the sequence fidelity of the microarray probes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.