Evidence map›Paper›PMID 23967430›Full record

ArticleIranian journal of public health2013

Molecular Genotyping of Toxoplasma gondii in Human Spontaneous Aborted Fetuses in Shiraz, Southern Iran.

Qasem Asgari, Maysam Fekri, Ahmad Monabati, Mohsen Kalantary, Iraj Mohammadpour, Mohammad Hossein Motazedian, Bahador Sarkari

Open access · greenAbstract read
In one paragraph

Article in Iranian journal of public health, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 1 pooled it
3.9field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 1 synthesis or guideline pooled it, 43 citations in OpenAlex.

  1. Is Toxoplasma gondii type related to clinical outcome in human congenital infection? Systematic and critical review.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016
    Pooled it
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  8. Detection and phylogenetic analysis ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022
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  9. Molecular and serological study on congenital toxoplasmosis in newborn of Shiraz, Southern Iran.Environmental science and pollution research international · 2021
    Article
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  11. Detection of Acute and ChronicJournal of parasitology research · 2021
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  12. Interdisciplinary perspectives on infectious diseases · 2018
    Article
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  15. Veterinary world · 2017
    Article
  16. Review
  17. Article
  18. Molecular Diagnosis of Toxoplasma gondii in Aborted Women.Jundishapur journal of microbiology · 2015
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Qasem AsgariDept. of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Maysam Fekri
Ahmad Monabati
Mohsen Kalantary
Iraj Mohammadpour
Mohammad Hossein Motazedian
Bahador Sarkari
Shiraz University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCongenital toxoplasmosis is associated with variable complications including encephalitis, microcephaly, hydrocephaly, hepatitis, lymphadenopathy and even intrauterine death. Presence of Toxoplasma gondii in human placenta may induce congenital infection. The aim of this study was to determine the genotypes of Toxoplasma gondii infection in human spontaneous aborted fetuses in Shiraz, south of Iran.

methodsFive hundred and forty two paraffin-embedded blocks of aborted placenta were collected, from two university-affiliated hospitals in Shiraz. Occurrence of spontaneous abortion was confirmed by examine of the slides. After re-cutting of the blocks and dewaxing, semi-nested PCR assay was used to detect the fragments of T. gondii B1 gene in the samples. Also direct molecular genotyping was performed on positive samples with Restriction Fragment Length Polymorphism-PCR analysis on the SAG2 gene.

resultsAmong the 542 tissue samples, the B1 gene was amplified from 78 (14.4%) of cases with the semi nested PCR and typed by RFLP. The genotype of Toxoplasma strains of 65 (out of 78) PCR-positive samples were evaluated and 54 out of 65 (83.1%) were found to be type II and 11 out of 65 (16.9%) were type I.

conclusionConsidering the high level of Toxoplasma infection in aborted fetuses in this study, Toxoplasma might largely contribute to spontaneous abortion in this area of Iran.

Indexed as

AbortionGenotypeIranToxoplasma gondii

Identifiers

PMID23967430
PMCPMC3744259
OpenAlexW2133126092

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.