ArticlePloS one2013
Characterization of GABAergic neurons in the mouse lateral septum: a double fluorescence in situ hybridization and immunohistochemical study using tyramide signal amplification.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 110 citations in OpenAlex.
- The Molecular Architecture of Somatic Spines of the Lateral Septum.The Journal of comparative neurology · 2026Article
- Lateral septal PACAP signaling regulates stress and anxiety reactions.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2026Article
- Mice produce interneurons in the septum as a response to aversive experiences and antidepressant treatment.Science advances · 2026Article
- Achieving cell-type specific transduction with adeno-associated viral vectors in pigeons.Current research in neurobiology · 2026Article
- Dopaminergic Innervation of the Nidopallium Caudolaterale in the Japanese Quail.The Journal of comparative neurology · 2026Article
- Bidirectional regulation of social memory by two distinct types of GABAergic neurons in the lateral septum.Nature communications · 2026Article
- Alcohol Facilitates Social Dominance Via Activating Dopamine 1 Receptor-expressing Neurons in the Lateral Septum of Male Mice.Molecular neurobiology · 2025Article
- The medial septum-hippocampus-lateral septum circuitry in spatial memory: linking healthy function to early Alzheimer's disease and translational opportunities.Translational neurodegeneration · 2025Review
- Lateral septal inhibition of nucleus basalis through direct and indirect pathways in focal limbic seizures.bioRxiv : the preprint server for biology · 2025Article
- Organization of Brainwide Inputs to Discrete Lateral Septum Projection Populations.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Ankyrin-G and Its Binding Partners in Neurons: Orchestrating the Molecular Structure of the Axon Initial Segment.Biomolecules · 2025Review
- Septo-hypothalamic regulation of binge-like alcohol consumption by the nociceptin system.Cell reports · 2025Article
- Auditory fear memory retrieval requires BLA-LS and LS-VMH circuitries via GABAergic and dopaminergic neurons.EMBO reports · 2025Article
- Transcriptomic characterization of human lateral septum neurons reveals conserved and divergent marker genes across species.iScience · 2025Article
- Dorsal raphe to basolateral amygdala corticotropin-releasing factor circuit regulates cocaine-memory reconsolidation.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2024Article
- Transcriptomic characterization of human lateral septum neurons reveals conserved and divergent marker genes across species.bioRxiv : the preprint server for biology · 2024Article
- A thalamic nucleus reuniens-lateral septum-lateral hypothalamus circuit for comorbid anxiety-like behaviors in chronic itch.Science advances · 2024Article
- Dorsal Raphe to Basolateral Amygdala Corticotropin-Releasing Factor Circuit Regulates Cocaine-Memory Reconsolidation.bioRxiv : the preprint server for biology · 2024Article
- TrkB-dependent regulation of molecular signaling across septal cell types.Translational psychiatry · 2024Article
- Oxytocin Receptor-Expressing Neurons in the Medial Preoptic Area Are Essential for Lactation, whereas Those in the Lateral Septum Are Not Critical for Maternal Behavior.Neuroendocrinology · 2024Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Gamma-aminobutyric acid (GABA) neurotransmission in the lateral septum (LS) is implicated in modulating various behavioral processes, including emotional reactivity and maternal behavior. However, identifying the phenotype of GABAergic neurons in the CNS has been hampered by the longstanding inability to reliably detect somal immunoreactivity for GABA or glutamic acid decarboxylase (GAD), the enzyme that produces GABA. In this study, we designed unique probes for both GAD65 (GAD2) and GAD67 (GAD1), and used fluorescence in Situ hybridization (FISH) with tyramide signal amplification (TSA) to achieve unequivocal detection of cell bodies of GABAergic neurons by GAD mRNAs. We quantitatively characterized the expression and chemical phenotype of GABAergic neurons across each subdivision of LS and in cingulate cortex (Cg) and medial preoptic area (MPOA) in female mice. Across LS, almost all GAD65 mRNA-expressing neurons were found to contain GAD67 mRNA (approximately 95-98%), while a small proportion of GAD67 mRNA-containing neurons did not express GAD65 mRNA (5-14%). Using the neuronal marker NeuN, almost every neuron in LS (> 90%) was also found to be GABA-positive. Interneuron markers using calcium-binding proteins showed that LS GABAergic neurons displayed immunoreactivity for calbindin (CB) or calretinin (CR), but not parvalbumin (PV); almost all CB- or CR-immunoreactive neurons (98-100%) were GABAergic. The proportion of GABAergic neurons immunoreactive for CB or CR varied depending on the subdivisions examined, with the highest percentage of colocalization in the caudal intermediate LS (LSI) (approximately 58% for CB and 35% for CR). These findings suggest that the vast majority of GABAergic neurons within the LS have the potential for synthesizing GABA via the dual enzyme systems GAD65 and GAD67, and each subtype of GABAergic neurons identified by distinct calcium-binding proteins may exert unique roles in the physiological function and neuronal circuitry of the LS.
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