ArticlePloS one2013
Prolonged minocycline treatment impairs motor neuronal survival and glial function in organotypic rat spinal cord cultures.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- From 2D to 3D Co-Culture Systems: A Review of Co-Culture Models to Study the Neural Cells Interaction.International journal of molecular sciences · 2022Review
- CX3CR1-Targeted PLGA Nanoparticles Reduce Microglia Activation and Pain Behavior in Rats with Spinal Nerve Ligation.International journal of molecular sciences · 2020Article
- Article
- Melanoma-Induced Reprogramming of Schwann Cell Signaling Aids Tumor Growth.Cancer research · 2019Article
- Cell viability in three ex vivo rat models of spinal cord injury.Journal of anatomy · 2019Article
- Intra-CA1 Administration of Minocycline Alters the Expression of Inflammation-Related Genes in Hippocampus of CCI Rats.Frontiers in molecular neuroscience · 2019Article
- The Effects of Minocycline on Spinal Root Avulsion Injury in Rat Model.The Malaysian journal of medical sciences : MJMS · 2017Article
- Vascular endothelial growth factor enhances axonal outgrowth in organotypic spinal cord slices via vascular endothelial growth factor receptor 1 and 2.Tissue engineering and regenerative medicine · 2016Article
- Organotypic Spinal Cord Culture: a Proper Platform for the Functional Screening.Molecular neurobiology · 2016Review
- Selected gene profiles of stressed NSC-34 cells and rat spinal cord following peripheral nerve reconstruction and minocycline treatment.Experimental and therapeutic medicine · 2016Article
- Growth factor choice is critical for successful functionalization of nanoparticles.Frontiers in neuroscience · 2015Article
- Microglial Activation Promotes Cell Survival in Organotypic Cultures of Postnatal Mouse Retinal Explants.PloS one · 2015Article
- Matrix metalloproteinase-2 is downregulated in sciatic nerve by streptozotocin induced diabetes and/or treatment with minocycline: Implications for nerve regeneration.Experimental neurology · 2014Article
- Delta-opioid receptor analgesia is independent of microglial activation in a rat model of neuropathic pain.PloS one · 2014Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMinocycline, a second-generation tetracycline antibiotic, exhibits anti-inflammatory and neuroprotective effects in various experimental models of neurological diseases, such as stroke, Alzheimer's disease, amyotrophic lateral sclerosis and spinal cord injury. However, conflicting results have prompted a debate regarding the beneficial effects of minocycline.
methodsIn this study, we analyzed minocycline treatment in organotypic spinal cord cultures of neonatal rats as a model of motor neuron survival and regeneration after injury. Minocycline was administered in 2 different concentrations (10 and 100 µM) at various time points in culture and fixed after 1 week.
resultsProlonged minocycline administration decreased the survival of motor neurons in the organotypic cultures. This effect was strongly enhanced with higher concentrations of minocycline. High concentrations of minocycline reduced the number of DAPI-positive cell nuclei in organotypic cultures and simultaneously inhibited microglial activation. Astrocytes, which covered the surface of the control organotypic cultures, revealed a peripheral distribution after early minocycline treatment. Thus, we further analyzed the effects of 100 µM minocycline on the viability and migration ability of dispersed primary glial cell cultures. We found that minocycline reduced cell viability, delayed wound closure in a scratch migration assay and increased connexin 43 protein levels in these cultures.
conclusionsThe administration of high doses of minocycline was deleterious for motor neuron survival. In addition, it inhibited microglial activation and impaired glial viability and migration. These data suggest that especially high doses of minocycline might have undesired affects in treatment of spinal cord injury. Further experiments are required to determine the conditions for the safe clinical administration of minocycline in spinal cord injured patients.
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