ArticlePloS one2013
ESTs analysis reveals putative genes involved in symbiotic seed germination in Dendrobium officinale.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- Deciphering Molecular Pathways ofMicroorganisms · 2026Article
- Symbiosis between Dendrobium catenatum protocorms and Serendipita indica involves the plant hypoxia response pathway.Plant physiology · 2023Article
- Bioactive steroids from seed germination supporting fungus (Mycology · 2023Article
- Effect of cultivation mode on bacterial and fungal communities of Dendrobium catenatum.BMC microbiology · 2022Article
- Genome-wide identification and characterization of active ingredients related β-Glucosidases in Dendrobium catenatum.BMC genomics · 2022Article
- Genome-Wide Analysis of theGenes · 2022Article
- Proteomic and Transcriptomic Analyses Indicate Metabolic Changes and Reduced Defense Responses in Mycorrhizal Roots ofJournal of fungi (Basel, Switzerland) · 2020Article
- Effect of different mycobionts on symbiotic germination and seedling growth of Dendrobium officinale, an important medicinal orchid.Botanical studies · 2020Article
- Functional Insights into the Roles of Hormones in theInternational journal of molecular sciences · 2018Article
- Comparative transcriptomics provides insight into the molecular basis of species diversification of section Trigonopedia (Cypripedium) on the Qinghai-Tibetan Plateau.Scientific reports · 2018Article
- Molecular characterization and expression analysis of WRKY family genes in Dendrobium officinale.Genes & genomics · 2018Article
- Immunolocalization and Changes of Hydroxyproline-Rich Glycoproteins During Symbiotic Germination ofFrontiers in plant science · 2018Article
- Transcriptomic analyses reveal clathrin-mediated endocytosis involved in symbiotic seed germination of Gastrodia elata.Botanical studies · 2017Article
- A perspective on orchid seed and protocorm development.Botanical studies · 2017Review
- Transcriptome Analysis of Genes Involved in Dendrobine Biosynthesis in Dendrobium nobile Lindl. Infected with Mycorrhizal Fungus MF23 (Mycena sp.).Scientific reports · 2017Article
- Functional insights into the late embryogenesis abundant (LEA) protein family from Dendrobium officinale (Orchidaceae) using an Escherichia coli system.Scientific reports · 2016Article
- Article
- Effect ofEvidence-based complementary and alternative medicine : eCAM · 2016Article
- Cytotoxic and Antifungal Constituents Isolated from the Metabolites of Endophytic Fungus DO14 from Dendrobium officinale.Molecules (Basel, Switzerland) · 2015Article
- Comparative Transcriptome Analysis of Genes Involved in GA-GID1-DELLA Regulatory Module in Symbiotic and Asymbiotic Seed Germination of Anoectochilus roxburghii (Wall.) Lindl. (Orchidaceae).International journal of molecular sciences · 2015Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dendrobiumofficinale (Orchidaceae) is one of the world's most endangered plants with great medicinal value. In nature, D. officinale seeds must establish symbiotic relationships with fungi to germinate. However, the molecular events involved in the interaction between fungus and plant during this process are poorly understood. To isolate the genes involved in symbiotic germination, a suppression subtractive hybridization (SSH) cDNA library of symbiotically germinated D. officinale seeds was constructed. From this library, 1437 expressed sequence tags (ESTs) were clustered to 1074 Unigenes (including 902 singletons and 172 contigs), which were searched against the NCBI non-redundant (NR) protein database (E-value cutoff, e(-5)). Based on sequence similarity with known proteins, 579 differentially expressed genes in D. officinale were identified and classified into different functional categories by Gene Ontology (GO), Clusters of orthologous Groups of proteins (COGs) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The expression levels of 15 selected genes emblematic of symbiotic germination were confirmed via real-time quantitative PCR. These genes were classified into various categories, including defense and stress response, metabolism, transcriptional regulation, transport process and signal transduction pathways. All transcripts were upregulated in the symbiotically germinated seeds (SGS). The functions of these genes in symbiotic germination were predicted. Furthermore, two fungus-induced calcium-dependent protein kinases (CDPKs), which were upregulated 6.76- and 26.69-fold in SGS compared with un-germinated seeds (UGS), were cloned from D. officinale and characterized for the first time. This study provides the first global overview of genes putatively involved in D. officinale symbiotic seed germination and provides a foundation for further functional research regarding symbiotic relationships in orchids.
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