ArticlePloS one2013
Structural basis for the interaction of the adaptor protein grb14 with activated ras.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Motif-guided identification of KRAS-interacting proteins.BMC biology · 2024Article
- Functional and structural insights into RAS effector proteins.Molecular cell · 2024Review
- ZEPPI: Proteome-scale sequence-based evaluation of protein-protein interaction models.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- HPLC method to resolve, identify and quantify guanine nucleotides bound to recombinant ras GTPase.Analytical biochemistry · 2021Article
- RAS interaction with Sin1 is dispensable for mTORC2 assembly and activity.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Regulation of Phosphoinositide Levels in the Retina by Protein Tyrosine Phosphatase 1B and Growth Factor Receptor-Bound Protein 14.Biomolecules · 2021Article
- Ras-guanine nucleotide complexes: A UV spectral deconvolution method to analyze protein concentration, nucleotide stoichiometry, and purity.Analytical biochemistry · 2021Article
- The Ins and Outs of RAS Effector Complexes.Biomolecules · 2021Review
- Engineering of Biological Pathways: Complex Formation and Signal Transduction.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The Plasma Membrane as a Competitive Inhibitor and Positive Allosteric Modulator of KRas4B Signaling.Biophysical journal · 2020Article
- The adaptor protein Ste50 directly modulates yeast MAPK signaling specificity through differential connections of its RA domain.Molecular biology of the cell · 2019Article
- Grb7 protein RA domain oligomerization.Journal of molecular recognition : JMR · 2017Article
- Drugging Ras GTPase: a comprehensive mechanistic and signaling structural view.Chemical Society reviews · 2016Review
- The RAS-Effector Interface: Isoform-Specific Differences in the Effector Binding Regions.PloS one · 2016Article
- The Function of Embryonic Stem Cell-expressed RAS (E-RAS), a Unique RAS Family Member, Correlates with Its Additional Motifs and Its Structural Properties.The Journal of biological chemistry · 2015Article
- The structure of Rap1 in complex with RIAM reveals specificity determinants and recruitment mechanism.Journal of molecular cell biology · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Grb14, a member of the Grb7-10-14 family of cytoplasmic adaptor proteins, is a tissue-specific negative regulator of insulin signaling. Grb7-10-14 contain several signaling modules, including a Ras-associating (RA) domain, a pleckstrin-homology (PH) domain, a family-specific BPS (between PH and SH2) region, and a C-terminal Src-homology-2 (SH2) domain. We showed previously that the RA and PH domains, along with the BPS region and SH2 domain, are necessary for downregulation of insulin signaling. Here, we report the crystal structure at 2.4-Å resolution of the Grb14 RA and PH domains in complex with GTP-loaded H-Ras (G12V). The structure reveals that the Grb14 RA and PH domains form an integrated structural unit capable of binding simultaneously to small GTPases and phosphoinositide lipids. The overall mode of binding of the Grb14 RA domain to activated H-Ras is similar to that of the RA domains of RalGDS and Raf1 but with important distinctions. The integrated RA-PH structural unit in Grb7-10-14 is also found in a second adaptor family that includes Rap1-interacting adaptor molecule (RIAM) and lamellipodin, proteins involved in actin-cytoskeleton rearrangement. The structure of Grb14 RA-PH in complex with H-Ras represents the first detailed molecular characterization of tandem RA-PH domains bound to a small GTPase and provides insights into the molecular basis for specificity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.