ArticlePloS one2013
Hexosamine biosynthesis is a possible mechanism underlying hypoxia's effects on lipid metabolism in human adipocytes.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Article
- A nexus of lipid andFrontiers in endocrinology · 2022Review
- Article
- Physiological Responses to Hypoxia on Triglyceride Levels.Frontiers in physiology · 2021Review
- Elucidating nanoscale mechanical properties of diabetic human adipose tissue using atomic force microscopy.Scientific reports · 2020Article
- Pathophysiological implications of hypoxia in human diseases.Journal of biomedical science · 2020Review
- Glutamine Analogues Impair Cell Proliferation, the Intracellular Cycle and Metacyclogenesis inMolecules (Basel, Switzerland) · 2020Article
- A Human 3D Extracellular Matrix-Adipocyte Culture Model for Studying Matrix-Cell Metabolic Crosstalk.Journal of visualized experiments : JoVE · 2019Article
- Diabetes-Specific Regulation of Adipocyte Metabolism by the Adipose Tissue Extracellular Matrix.The Journal of clinical endocrinology and metabolism · 2017Article
- Differentiation and Metabolic Interrogation of Human Adipocytes.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Metabolic Reprogramming by Hexosamine Biosynthetic and Golgi N-Glycan Branching Pathways.Scientific reports · 2016Article
- Limited OXPHOS capacity in white adipocytes is a hallmark of obesity in laboratory mice irrespective of the glucose tolerance status.Molecular metabolism · 2015Article
- Alveolar-capillary adaptation to chronic hypoxia in the fatty lung.Acta physiologica (Oxford, England) · 2015Article
- Oxygen deprivation and the cellular response to hypoxia in adipocytes - perspectives on white and brown adipose tissues in obesity.Frontiers in endocrinology · 2015Review
- Cell-autonomous heterogeneity of nutrient uptake in white adipose tissue of rhesus macaques.Endocrinology · 2015Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
introductionHypoxia regulates adipocyte metabolism. Hexosamine biosynthesis is implicated in murine 3T3L1 adipocyte differentiation and is a possible underlying mechanism for hypoxia's effects on adipocyte metabolism.
methodsLipid metabolism was studied in human visceral and subcutaneous adipocytes in in vitro hypoxic culture with adipophilic staining, glycerol release, and palmitate oxidation assays. Gene expression and hexosamine biosynthesis activation was studied with QRTPCR, immunofluorescence microscopy, and Western blotting.
resultsHypoxia inhibits lipogenesis and induces basal lipolysis in visceral and subcutaneous human adipocytes. Hypoxia induces fatty acid oxidation in visceral adipocytes but had no effect on fatty acid oxidation in subcutaneous adipocytes. Hypoxia inhibits hexosamine biosynthesis in adipocytes. Inhibition of hexosamine biosynthesis with azaserine attenuates lipogenesis and induces lipolysis in adipocytes in normoxic conditions, while promotion of hexosamine biosynthesis with glucosamine in hypoxic conditions slightly increases lipogenesis.
conclusionsHypoxia's net effect on human adipocyte lipid metabolism would be expected to impair adipocyte buffering capacity and contribute to systemic lipotoxicity. Our data suggest that hypoxia may mediate its effects on lipogenesis and lipolysis through inhibition of hexosamine biosynthesis. Hexosamine biosynthesis represents a target for manipulation of adipocyte metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.