ArticlePloS one2013
Bilateral nerve alterations in a unilateral experimental neurotrophic keratopathy model: a lateral conjunctival approach for trigeminal axotomy.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 54 citations in OpenAlex.
- A novel rat model of neurotrophic keratopathy complicated with limbal stem cell deficiency induced by trigeminal ophthalmic branch injury.BMC ophthalmology · 2026Article
- Corneal epithelial cells upregulate macropinocytosis to engulf metabolically active axonal mitochondria released by injured axons.The ocular surface · 2025Article
- Corneal Sensory Denervation Causes Epithelial Ferroptosis and Delayed Healing in Mice.Investigative ophthalmology & visual science · 2025Article
- Bilateral In Vivo Confocal Microscopic Changes of the Corneal Subbasal Nerve Plexus in Patients with Acute Herpes Zoster Ophthalmicus.Ophthalmology and therapy · 2025Article
- Modeling Neuropathic Corneal Pain: Pulled Nerve Approach With Elevated Krt16 Gene Expression.Investigative ophthalmology & visual science · 2025Article
- Disrupted Mitochondrial Dynamics Impair Corneal Epithelial Healing in Neurotrophic Keratopathy.International journal of molecular sciences · 2025Article
- CGRP Released by Corneal Sensory Nerve Maintains Tear Secretion of the Lacrimal Gland.Investigative ophthalmology & visual science · 2024Article
- Chitosan-based thermosensitive hydrogel with long-term release of murine nerve growth factor for neurotrophic keratopathy.Neural regeneration research · 2024Article
- Establishing a Mouse Model of Chlorpromazine-Induced Corneal Trigeminal Denervation.Translational vision science & technology · 2023Article
- Trigeminal nerve-derived substance P regulates limbal stem cells by the PI3K-AKT pathway.iScience · 2023Article
- Immunomodulatory Role of Neuropeptides in the Cornea.Biomedicines · 2022Review
- Effect of herpes simplex keratitis scar location on bilateral corneal nerve alterations: an in vivo confocal microscopy study.The British journal of ophthalmology · 2022Article
- Prolonged Retrobulbar Local Anesthesia of the Cornea Does Not Cause Keratopathy in Mice.Translational vision science & technology · 2022Article
- Concurrent ocular pain in patients with neurotrophic keratopathy.The ocular surface · 2021Article
- Unilateral Corneal Insult Also Alters Sensory Nerve Activity in the Contralateral Eye.Frontiers in medicine · 2021Article
- The impact of euthanasia and enucleation on mouse corneal epithelial axon density and nerve terminal morphology.The ocular surface · 2020Article
- Corneal pain and experimental model development.Progress in retinal and eye research · 2019Review
- Corneal Sub-Basal Nerve Changes in Patients with Herpetic Keratitis During Acute Phase and after 6 Months.Medicina (Kaunas, Lithuania) · 2019Article
- [Pathogenesis and epidemiology of neurotrophic keratopathy].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2019Review
- Corneal esthesiometry and sub-basal nerves morphological changes in herpes simplex virus keratitis/uveitis patients.International journal of ophthalmology · 2019Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
Abstract
To study bilateral nerve changes in a newly developed novel mouse model for neurotrophic keratopathy by approaching the trigeminal nerve from the lateral fornix. Surgical axotomy of the ciliary nerve of the trigeminal nerve was performed in adult BALB/c mice at the posterior sclera. Axotomized, contralateral, and sham-treated corneas were excised on post-operative days 1, 3, 5, 7 and 14 and immunofluorescence histochemistry was performed with anti-β-tubulin antibody to evaluate corneal nerve density. Blink reflex was evaluated using a nylon thread. The survival rate was 100% with minimal bleeding during axotomy and a surgical time of 8±0.5 minutes. The blink reflex was diminished at day 1 after axotomy, but remained intact in the contralateral eyes in all mice. The central and peripheral subbasal nerves were not detectable in the axotomized cornea at day 1 (p<0.001), compared to normal eyes (101.3±14.8 and 69.7±12.0 mm/mm² centrally and peripherally). Interestingly, the subbasal nerve density in the contralateral non-surgical eyes also decreased significantly to 62.4±2.8 mm/mm² in the center from day 1 (p<0.001), but did not change in the periphery (77.3±11.7 mm/mm², P = 0.819). Our novel trigeminal axotomy mouse model is highly effective, less invasive, rapid, and has a high survival rate, demonstrating immediate loss of subbasal nerves in axotomized eyes and decreased subbasal nerves in contralateral eyes after unilateral axotomy. This model will allow investigating the effects of corneal nerve damage and serves as a new model for neurotrophic keratopathy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.