ArticlePLoS genetics2013
Genetic circuits that govern bisexual and unisexual reproduction in Cryptococcus neoformans.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 79 citations in OpenAlex.
- Genomic hallmarks of parasexual reproduction in three hybrid groups of the human pathogenbioRxiv : the preprint server for biology · 2026Article
- The Zinc Finger Protein Zfp2 Regulates Cell-Cell Fusion and Virulence inJournal of fungi (Basel, Switzerland) · 2025Article
- Distinct evolutionary trajectories following loss of RNA interference inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Distinct evolutionary trajectories following loss of RNA interference inbioRxiv : the preprint server for biology · 2024Article
- Biogenesis, germination, and pathogenesis ofMicrobiology and molecular biology reviews : MMBR · 2024Review
- Genetic Transformation of Cryptococcus Species with Agrobacterium Transfer DNA.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Ubiquitin Degradation of the AICAR Transformylase/IMP Cyclohydrolase Ade16 Regulates the Sexual Reproduction ofJournal of fungi (Basel, Switzerland) · 2023Article
- Regulatory basis for reproductive flexibility in a meningitis-causing fungal pathogen.Nature communications · 2022Article
- Uncontrolled transposition following RNAi loss causes hypermutation and antifungal drug resistance in clinical isolates of Cryptococcus neoformans.Nature microbiology · 2022Article
- A Velvet Transcription Factor Specifically Activates Mating through a Novel Mating-Responsive Protein in the Human Fungal Pathogen Cryptococcus deneoformans.Microbiology spectrum · 2022Article
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- Gluconate Kinase Is Required for Gluconate Assimilation and Sporulation in Cryptococcus neoformans.Microbiology spectrum · 2022Article
- Epistatic genetic interactions govern morphogenesis during sexual reproduction and infection in a global human fungal pathogen.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A unique cell wall synthetic response evoked by glucosamine determines pathogenicity-associated fungal cellular differentiation.PLoS genetics · 2021Article
- Mating Systems in True Morels (Microbiology and molecular biology reviews : MMBR · 2021Review
- Cryptococcus neoformans: Sex, morphogenesis, and virulence.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2021Review
- Factors enforcing the species boundary between the human pathogens Cryptococcus neoformans and Cryptococcus deneoformans.PLoS genetics · 2021Article
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- The Pheromone and Pheromone Receptor Mating-Type Locus Is Involved in Controlling Uniparental Mitochondrial Inheritance inGenetics · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Cryptococcus neoformans is a human fungal pathogen with a defined sexual cycle. Nutrient-limiting conditions and pheromones induce a dimorphic transition from unicellular yeast to multicellular hyphae and the production of infectious spores. Sexual reproduction involves cells of either opposite (bisexual) or one (unisexual) mating type. Bisexual and unisexual reproduction are governed by shared components of the conserved pheromone-sensing Cpk1 MAPK signal transduction cascade and by Mat2, the major transcriptional regulator of the pathway. However, the downstream targets of the pathway are largely unknown, and homology-based approaches have failed to yield downstream transcriptional regulators or other targets. In this study, we applied insertional mutagenesis via Agrobacterium tumefaciens transkingdom DNA delivery to identify mutants with unisexual reproduction defects. In addition to elements known to be involved in sexual development (Crg1, Ste7, Mat2, and Znf2), three key regulators of sexual development were identified by our screen: Znf3, Spo11, and Ubc5. Spo11 and Ubc5 promote sporulation during both bisexual and unisexual reproduction. Genetic and phenotypic analyses provide further evidence implicating both genes in the regulation of meiosis. Phenotypic analysis of sexual development showed that Znf3 is required for hyphal development during unisexual reproduction and also plays a central role during bisexual reproduction. Znf3 promotes cell fusion and pheromone production through a pathway parallel to and independent of the pheromone signaling cascade. Surprisingly, Znf3 participates in transposon silencing during unisexual reproduction and may serve as a link between RNAi silencing and sexual development. Our studies illustrate the power of unbiased genetic screens to reveal both novel and conserved circuits that operate sexual reproduction.
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