ArticlePLoS genetics2013
Introns regulate gene expression in Cryptococcus neoformans in a Pab2p dependent pathway.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 51 citations in OpenAlex.
- Omics-based dissection of alternative splicing and the regulatory architecture in fungal genomes.Current research in microbial sciences · 2026Review
- Genetically Encoded Sensors for Monitoring Intracellular Redox Health of the Pathogenic FungusACS sensors · 2025Article
- Introns increase gene expression in Caenorhabditis elegans by a mechanism that must be at least partly different than in plants.Scientific reports · 2025Article
- A Chimeric ORF Fusion Phenotypic Reporter forJournal of fungi (Basel, Switzerland) · 2024Article
- Alternative TSS use is widespread in Cryptococcus fungi in response to environmental cues and regulated genome-wide by the transcription factor Tur1.PLoS biology · 2024Article
- Cryptococcus neoformans Slu7 ensures nuclear positioning during mitotic progression through RNA splicing.PLoS genetics · 2024Article
- A Landscape of the Genomic Structure ofJournal of fungi (Basel, Switzerland) · 2023Article
- Intron retention coupled with nonsense-mediated decay is involved in cellulase biosynthesis in cellulolytic fungi.Biotechnology for biofuels and bioproducts · 2022Article
- Genetic Variations in Metallothionein Genes and Susceptibility to Hypertensive Disorders of Pregnancy: A Case-Control Study.Frontiers in genetics · 2022Article
- Proximity to the Promoter and Terminator Regions Regulates the Transcription Enhancement Potential of an Intron.Frontiers in molecular biosciences · 2021Article
- Article
- Quantitative global studies reveal differential translational control by start codon context across the fungal kingdom.Nucleic acids research · 2020Article
- Sensitive bioluminescence imaging of fungal dissemination to the brain in mouse models of cryptococcosis.Disease models & mechanisms · 2019Article
- TheFrontiers in plant science · 2019Article
- Introns as Gene Regulators: A Brick on the Accelerator.Frontiers in genetics · 2018Article
- Introns in Cryptococcus.Memorias do Instituto Oswaldo Cruz · 2018Review
- Article
- Intron retention-dependent gene regulation in Cryptococcus neoformans.Scientific reports · 2016Article
- Viral Evasion and Manipulation of Host RNA Quality Control Pathways.Journal of virology · 2016Review
- A native promoter and inclusion of an intron is necessary for efficient expression of GFP or mRFP in Armillaria mellea.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most Cryptococccus neoformans genes are interrupted by introns, and alternative splicing occurs very often. In this study, we examined the influence of introns on C. neoformans gene expression. For most tested genes, elimination of introns greatly reduces mRNA accumulation. Strikingly, the number and the position of introns modulate the gene expression level in a cumulative manner. A screen for mutant strains able to express functionally an intronless allele revealed that the nuclear poly(A) binding protein Pab2 modulates intron-dependent regulation of gene expression in C. neoformans. PAB2 deletion partially restored accumulation of intronless mRNA. In addition, our results demonstrated that the essential nucleases Rrp44p and Xrn2p are implicated in the degradation of mRNA transcribed from an intronless allele in C. neoformans. Double mutant constructions and over-expression experiments suggested that Pab2p and Xrn2p could act in the same pathway whereas Rrp44p appears to act independently. Finally, deletion of the RRP6 or the CID14 gene, encoding the nuclear exosome nuclease and the TRAMP complex associated poly(A) polymerase, respectively, has no effect on intronless allele expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.