ArticlePLoS genetics2013
Mediator directs co-transcriptional heterochromatin assembly by RNA interference-dependent and -independent pathways.
Article in PLoS genetics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Transcription-replication conflict resolution by nuclear RNA interference.Molecular cell · 2025Article
- Emerging Roles of Repetitive and Repeat-Containing RNA in Nuclear and Chromatin Organization and Gene Expression.Frontiers in cell and developmental biology · 2021Review
- A functional genetic screen identifies the Mediator complex as essential for SSX2-induced senescence.Cell death & disease · 2019Article
- H3K14 ubiquitylation promotes H3K9 methylation for heterochromatin assembly.EMBO reports · 2019Article
- RNA-induced initiation of transcriptional silencing (RITS) complex structure and function.RNA biology · 2019Review
- How does Hsp90 function in RNAi-dependent heterochromatin assembly?Current genetics · 2019Review
- A role for MED14 and UVH6 in heterochromatin transcription upon destabilization of silencing.Life science alliance · 2018Article
- A Heterochromatin Domain Forms Gradually at a New Telomere and Is Dynamic at Stable Telomeres.Molecular and cellular biology · 2018Article
- RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces pombe requires heat-shock molecular chaperones Hsp90 and Mas5.Epigenetics & chromatin · 2018Article
- The inhibition of checkpoint activation by telomeres does not involve exclusion of dimethylation of histone H4 lysine 20 (H4K20me2).F1000Research · 2018Article
- Selective termination of lncRNA transcription promotes heterochromatin silencing and cell differentiation.The EMBO journal · 2017Article
- A meiosis-specific Spt5 homolog involved in non-coding transcription.Nucleic acids research · 2017Article
- H3K36 methylation state and associated silencing mechanisms.Transcription · 2017Article
- Histone H3K36 trimethylation is essential for multiple silencing mechanisms in fission yeast.Nucleic acids research · 2016Article
- An Integrated Genomic Strategy Delineates Candidate Mediator Genes Regulating Grain Size and Weight in Rice.Scientific reports · 2016Article
- The Mediator complex: a central integrator of transcription.Nature reviews. Molecular cell biology · 2015Review
- Importance of Mediator complex in the regulation and integration of diverse signaling pathways in plants.Frontiers in plant science · 2015Review
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Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterochromatin at the pericentromeric repeats in fission yeast is assembled and spread by an RNAi-dependent mechanism, which is coupled with the transcription of non-coding RNA from the repeats by RNA polymerase II. In addition, Rrp6, a component of the nuclear exosome, also contributes to heterochromatin assembly and is coupled with non-coding RNA transcription. The multi-subunit complex Mediator, which directs initiation of RNA polymerase II-dependent transcription, has recently been suggested to function after initiation in processes such as elongation of transcription and splicing. However, the role of Mediator in the regulation of chromatin structure is not well understood. We investigated the role of Mediator in pericentromeric heterochromatin formation and found that deletion of specific subunits of the head domain of Mediator compromised heterochromatin structure. The Mediator head domain was required for Rrp6-dependent heterochromatin nucleation at the pericentromere and for RNAi-dependent spreading of heterochromatin into the neighboring region. In the latter process, Mediator appeared to contribute to efficient processing of siRNA from transcribed non-coding RNA, which was required for efficient spreading of heterochromatin. Furthermore, the head domain directed efficient transcription in heterochromatin. These results reveal a pivotal role for Mediator in multiple steps of transcription-coupled formation of pericentromeric heterochromatin. This observation further extends the role of Mediator to co-transcriptional chromatin regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.