Evidence map›Paper›PMID 23966396›Full record

ArticleJournal of virology2013

Varicella-zoster virus open reading frame 48 encodes an active nuclease.

Niklaus H Mueller, Don Gilden, Randall J Cohrs

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Niklaus H MuellerDepartments of Ophthalmology.
Don Gilden
Randall J Cohrs
University of Colorado Denver · US

Funding

VARICELLA ZOSTER VIRUS VASCULOPATHY: CHANGING CLINICAL PRACTICEP01AG032958 · NIA · UNIVERSITY OF COLORADO DENVER · PI COHRS, RANDALL J. · 2009 to 2023
$30.3M
NEUROBIOLOGY OF VARICELLA-ZOSTER VIRUSR01AG006127 · NIA · UNIVERSITY OF COLORADO DENVER · PI GILDEN, DONALD · 1987 to 2014
$4.2M
NEUROVIROLOGY-MOLECULAR BIOLOGYT32NS007321 · NINDS · UNIVERSITY OF COLORADO DENVER · PI GILDEN, DONALD · 1988 to 2014
$2.3M
NEUROBIOLOGY OF VARICELLA ZOSTER VIRUSR37AG006127 · NIA · UNIVERSITY OF COLORADO DENVER · PI GILDEN, DONALD H · 1995 to 2004
$2.3M
Prevention of VZV ReactivationR01NS082228 · NINDS · UNIVERSITY OF COLORADO DENVER · PI COHRS, RANDALL J. · 2013 to 2017
$1.7M
NIA NIH HHS AG006127NIA NIH HHS AG032958NIA NIH HHS P01 AG032958NIA NIH HHS R01 AG006127NIA NIH HHS R37 AG006127NINDS NIH HHS NS007321NINDS NIH HHS NS082228NINDS NIH HHS R01 NS082228NINDS NIH HHS T32 NS007321
6 · The paper itself

Abstract

Based on a DNA sequence and relative genomic position similar to those other herpesviruses, varicella-zoster virus (VZV) open reading frame 48 (ORF48) is predicted to encode an alkaline nuclease. Here we report the cloning, expression, purification, and characterization of recombinant VZV ORF48 protein and a VZV ORF48 point mutation (T172P). Protein encoded by wild-type ORF48, but not mutant protein, displayed both endo- and exonuclease activity, identifying ORF48 as a potential therapeutic target in VZV disease since efficient viral replication requires viral nuclease activity.

Indexed as

Amino Acid SequenceCloning, MolecularGene ExpressionHerpesvirus 3, HumanMolecular Sequence DataMutant ProteinsMutation, MissenseRecombinant ProteinsRibonucleasesSequence AlignmentViral Nonstructural ProteinsMutant ProteinsRecombinant ProteinsRibonucleasesviral alkaline nucleaseViral Nonstructural Proteins

Identifiers

PMID23966396
PMCPMC3807315
OpenAlexW2015044356

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.