ArticleThe ISME journal2014
Genome sequencing of a single cell of the widely distributed marine subsurface Dehalococcoidia, phylum Chloroflexi.
Article in The ISME journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 178 citations in OpenAlex.
- Vertical distribution and metabolic diversity of autotrophic microbes in the deep sediment of the challenger deep.Environmental microbiome · 2026Article
- Innovations in Silicon Transport Shaped the Rise of Biosilicification and Skeletal Evolution in Sponges.Molecular biology and evolution · 2026Article
- Sedimentary hiatus causes abrupt decline and shifts in marine subsurface sediment microbial communities: a study from IODP Exp. 378 Site U1553 offshore southern New Zealand.FEMS microbiology letters · 2026Article
- Benthic Microbial Community Features and Environmental Correlates in the Northwest Pacific Polymetallic Nodule Field, with Comparative Analysis Across the Pacific.Microorganisms · 2026Article
- Chloroflexota in agricultural soils: current knowledge and future research directions.Frontiers in microbiology · 2026Review
- Multiscale Patterns of Bacterial and Protist Diversity Across Red Sea Coral Reefs.Microorganisms · 2025Article
- Genome-resolved transcriptomics reveals novel PCE-dehalogenating bacteria from Aarhus Bay sediments.mSystems · 2025Article
- Laminarin stimulates single cell rates of sulfate reduction whereas oxygen inhibits transcriptomic activity in coastal marine sediment.The ISME journal · 2025Article
- Vertically stratified carbon fixation and coupling processes in deep-sea sediment.ISME communications · 2025Article
- Heterogeneity of the horizontal environment drives community assemblages and species coexistence of prokaryotic communities in cold seep sediments.Frontiers in microbiology · 2025Article
- Genomic and biogeographic characterisation of the novel prasinovirus Mantoniella tinhauana virus 1.Environmental microbiology reports · 2024Article
- A comprehensive overview of the Chloroflexota community in wastewater treatment plants worldwide.mSystems · 2023Review
- Enrichment of Aerobic and Anaerobic Hydrocarbon-Degrading Bacteria from Multicontaminated Marine Sediment in Mar Piccolo Site (Taranto, Italy).Microorganisms · 2023Article
- Phylogenetically and metabolically diverse autotrophs in the world's deepest blue hole.ISME communications · 2023Article
- Taxonomic Re-Classification and Expansion of the Phylum Chloroflexota Based on over 5000 Genomes and Metagenome-Assembled Genomes.Microorganisms · 2023Article
- Long-read assembled metagenomic approaches improve our understanding on metabolic potentials of microbial community in mangrove sediments.Microbiome · 2023Article
- Cultivation of marine bacteria of the SAR202 clade.Nature communications · 2023Article
- Assessment of prokaryotic communities in Southwestern Atlantic deep-sea sediments reveals prevalent methanol-oxidising Methylomirabilales.Scientific reports · 2023Article
- Thermophilic Dehalococcoidia with unusual traits shed light on an unexpected past.The ISME journal · 2023Article
- Anaerobic degradation of organic carbon supports uncultured microbial populations in estuarine sediments.Microbiome · 2023Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria of the class Dehalococcoidia (DEH), phylum Chloroflexi, are widely distributed in the marine subsurface, yet metabolic properties of the many uncultivated lineages are completely unknown. This study therefore analysed genomic content from a single DEH cell designated 'DEH-J10' obtained from the sediments of Aarhus Bay, Denmark. Real-time PCR showed the DEH-J10 phylotype was abundant in upper sediments but was absent below 160 cm below sea floor. A 1.44 Mbp assembly was obtained and was estimated to represent up to 60.8% of the full genome. The predicted genome is much larger than genomes of cultivated DEH and appears to confer metabolic versatility. Numerous genes encoding enzymes of core and auxiliary beta-oxidation pathways were identified, suggesting that this organism is capable of oxidising various fatty acids and/or structurally related substrates. Additional substrate versatility was indicated by genes, which may enable the bacterium to oxidise aromatic compounds. Genes encoding enzymes of the reductive acetyl-CoA pathway were identified, which may also enable the fixation of CO2 or oxidation of organics completely to CO2. Genes encoding a putative dimethylsulphoxide reductase were the only evidence for a respiratory terminal reductase. No evidence for reductive dehalogenase genes was found. Genetic evidence also suggests that the organism could synthesise ATP by converting acetyl-CoA to acetate by substrate-level phosphorylation. Other encoded enzymes putatively conferring marine adaptations such as salt tolerance and organo-sulphate sulfohydrolysis were identified. Together, these analyses provide the first insights into the potential metabolic traits that may enable members of the DEH to occupy an ecological niche in marine sediments.
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