ArticleInternational journal of molecular sciences2013
Effects of reduced prolamin on seed storage protein composition and the nutritional quality of rice.
Article in International journal of molecular sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 49 citations in OpenAlex.
- Transcriptome-Based Identification of Novel Transcription Factors Regulating Seed Storage Proteins in Rice.Plants (Basel, Switzerland) · 2025Article
- Multiplex CRISPR/Cas9 Editing of Rice Prolamin and GluA Glutelin Genes Reveals Subfamily-Specific Effects on Seed Protein Composition.Plants (Basel, Switzerland) · 2025Article
- Compensatory Modulation of Seed Storage Protein Synthesis and Alteration of Starch Accumulation by Selective Editing of 13 kDa Prolamin Genes by CRISPR-Cas9 in Rice.International journal of molecular sciences · 2024Article
- Down-Regulation of Rice Glutelin byInternational journal of molecular sciences · 2023Article
- Engineering a feedback inhibition-insensitive plant dihydrodipicolinate synthase to increase lysine content in Camelina sativa seeds.Transgenic research · 2022Article
- Breeding Canola (Plants (Basel, Switzerland) · 2021Review
- Unique Glutelin Expression Patterns and Seed Endosperm Structure Facilitate Glutelin Accumulation in Polyploid Rice Seed.Rice (New York, N.Y.) · 2021Article
- Proteomic analysis of 2-chloroethanol extracts of rice (Food chemistry. Molecular sciences · 2020Article
- Proteomic analysis of reserve proteins in commercial rice cultivars.Food science & nutrition · 2020Article
- Advances in the Biology of Seed and Vegetative Storage Proteins Based on Two-Dimensional Electrophoresis Coupled to Mass Spectrometry.Molecules (Basel, Switzerland) · 2018Review
- Overexpression of the 16-kDa α-amylase/trypsin inhibitor RAG2 improves grain yield and quality of rice.Plant biotechnology journal · 2017Article
- Improving protein content and quality by over-expressing artificially synthetic fusion proteins with high lysine and threonine constituent in rice plants.Scientific reports · 2016Article
- Development of high-lysine rice via endosperm-specific expression of a foreign LYSINE RICH PROTEIN gene.BMC plant biology · 2016Article
- RNA Interference-Mediated Simultaneous Suppression of Seed Storage Proteins in Rice Grains.Frontiers in plant science · 2016Article
- Lack of Globulin Synthesis during Seed Development Alters Accumulation of Seed Storage Proteins in Rice.International journal of molecular sciences · 2015Article
- Biofortification of rice with lysine using endogenous histones.Plant molecular biology · 2015Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rice seed storage proteins accumulate in two types of protein body (PB-I and PB-II) that are nutrient sources for animals. PB-I is indigestible and negatively affects rice protein quality. To improve the nutritional value of rice seeds we are aiming to engineer the composition and accumulation of endogenous seed storage proteins. In this study we generated transgenic rice plants in which 13 kD prolamin genes were suppressed by RNA interference (13 kD pro-RNAi). Analysis based on qRT-PCR confirmed that the targeted 13 kD prolamins were markedly suppressed, and were compensated for by an increase in other storage proteins including 10 kD prolamin, glutelins, and chaperone proteins. The storage protein profiles further revealed that the levels of 13 kD prolamins were significantly reduced, while that of the glutelin precursor was slightly increased and the remaining storage proteins did not change. Amino acid analysis showed that the reduction of 13 kD prolamins resulted in a 28% increase in the lysine content relative to the wild type, indicating that the 13 kD pro-RNAi rice seeds are more nutritious. Furthermore, a reduction in the levels of 13 kD prolamins resulted in abnormal formation of PB-I, which was small and had no lamellar structure. These results suggest that alteration of prolamins can contribute to improving the nutritional quality of rice.
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