Evidence map›Paper›PMID 23965341›Full record

ArticleACS applied materials & interfaces2013

Spatial control of cell transfection using soluble or solid-phase redox agents and a redox-active ferrocenyl lipid.

Burcu S Aytar, John P E Muller, Yukishige Kondo, Nicholas L Abbott, David M Lynn

Abstract readLetter
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS applied materials & interfaces, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Burcu S AytarDepartment of Chemical and Biological Engineering, 1415 Engineering Drive, and §Department of Chemistry, 1101 University Avenue, University of Wisconsin-Madison , Madison, Wisconsin 53706, United States.
John P E Muller
Yukishige Kondo
Nicholas L Abbott
David M Lynn

Funding

Controlled Release of DNA from SurfacesR01EB006820 · NIBIB · UNIVERSITY OF WISCONSIN-MADISON · PI LYNN, DAVID M · 2008 to 2011
$1.3M
Spatial and Temporal Control of Transfection Using Ferrocene-Containing LipidsR21EB006168 · NIBIB · UNIVERSITY OF WISCONSIN-MADISON · PI LYNN, DAVID M · 2007 to 2008
$387k
NIBIB NIH HHS EB006168NIBIB NIH HHS EB006820NIBIB NIH HHS R01 EB006820NIBIB NIH HHS R21 EB006168
6 · The paper itself

Abstract

We report principles for active, user-defined control over the locations and timing with which DNA is expressed in cells. Our approach exploits unique properties of a ferrocenyl cationic lipid that is inactive when oxidized, but active when chemically reduced. We show that methods that exert spatial control over the administration of reducing agents can lead to local activation of lipoplexes and spatial control over gene expression. The versatility of this approach is demonstrated using both soluble and solid-phase reducing agents. These methods provide control over cell transfection, including methods for remote activation and the patterning of expression using solid-phase redox agents, that are difficult to achieve using conventional lipoplexes.

Indexed as

AnimalsChlorocebus aethiopsCOS CellsFerrous CompoundsGreen Fluorescent ProteinsLipidsMetallocenesOxidation-ReductionPhosphinesPlasmidsTransfectionferroceneFerrous CompoundsGreen Fluorescent ProteinsLipidsMetallocenesPhosphinestris(2-carboxyethyl)phosphine

Identifiers

PMID23965341
PMCPMC3789068

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.