ArticleAnalytical chemistry2013
Isolation of alpaca anti-idiotypic heavy-chain single-domain antibody for the aflatoxin immunoassay.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Gold nanoclusters-mediated enzyme cascade-amplified immunoassay for dual-mode sensing of aflatoxins.Mikrochimica acta · 2026Article
- A novel sustainable immunoassay for sensitive detection of atrazine based on the anti-idiotypic nanobody and recombinant full-length antibody.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2024Article
- Anti-Idiotypic Nanobodies Mimicking an Epitope of the Needle Protein of the Chlamydial Type III Secretion System for Targeted Immune Stimulation.International journal of molecular sciences · 2024Article
- Development of a sandwich ELISA for the specific quantitation of hemagglutinin (HA)-tagged proteins during their inducible expression in Escherichia coli.Analytical and bioanalytical chemistry · 2023Article
- Emerging Landscape of Nanobodies and Their Neutralizing Applications against SARS-CoV-2 Virus.ACS pharmacology & translational science · 2023Review
- M13 phage: a versatile building block for a highly specific analysis platform.Analytical and bioanalytical chemistry · 2023Review
- Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library.Molecules (Basel, Switzerland) · 2021Review
- Article
- Camelid Single-Domain Antibodies for the Development of Potent Diagnosis Platforms.Molecular diagnosis & therapy · 2021Review
- Enzyme cascade-amplified immunoassay based on the nanobody-alkaline phosphatase fusion and MnORSC advances · 2021Article
- Advances in Antibody Preparation Techniques for Immunoassays of Total Aflatoxin in Food.Molecules (Basel, Switzerland) · 2020Review
- Article
- Article
- Development of an Anti-Idiotypic VHH Antibody and Toxin-Free Enzyme Immunoassay for Ochratoxin A in Cereals.Toxins · 2019Article
- Small Molecular Contaminant and Microorganism Can Be Simultaneously Detected Based on Nanobody-Phage: Using Carcinogen Aflatoxin and Its Main FungalFrontiers in microbiology · 2019Article
- Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins.Frontiers in chemistry · 2019Article
- Nanobody Technology for Mycotoxin Detection in the Field of Food Safety: Current Status and Prospects.Toxins · 2018Review
- VHH Antibodies: Reagents for Mycotoxin Detection in Food Products.Sensors (Basel, Switzerland) · 2018Review
- Phage-Mediated Competitive Chemiluminescent Immunoassay for Detecting Cry1Ab Toxin by Using an Anti-Idiotypic Camel Nanobody.Journal of agricultural and food chemistry · 2018Article
- Determination of Aspergillus pathogens in agricultural products by a specific nanobody-polyclonal antibody sandwich ELISA.Scientific reports · 2017Article
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9 authors.
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Abstract
Anti-idiotypic antibodies recognize the antigenic determinants of an antibody, thus they can be used as surrogate antigens. Single-domain antibodies from camlid heavy-chain antibodies with the benefit features of small size, thermostability, and ease in expression, are leading candidates to produce anti-idiotypic antibodies. In this work, we constructed an antibody phage library from the mRNA of an alpaca immunized with an antiaflatoxin monoclonal antibody (mAb) 1C11. Three anti-idiotypic VHH antibodies were isolated and applied to immunoassay toward aflatoxin as a coating antigen. The best immunoassay developed with one of these VHH antibodies shows an IC50 of 0.16 ng/mL toward aflatoxin B1 and cross-reactivity toward aflatoxin B2, G1, and G2 of 90.4%, 54.4%, and 37.7%, respectively. The VHH-based immunoassay was successfully applied to the analysis of peanuts, corn, and rice, which are the predominant commodities regularly contaminated by aflatoxins. A good correlation (r(2) = 0.89) was found between the data obtained from the conventional ELISA and the ELISA based on a VHH coating antigen for the analysis of aflatoxins in peanuts and feedstuff. The use of biotechnology in developing the surrogate, the absence of standard aflatoxin and organic solvents in the synthesis procedures, and the reproducibility of the VHH antibody makes it an ideal strategy for replacing conventional synthesized antigens.
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