ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2013
De novo Ixodes ricinus salivary gland transcriptome analysis using two next-generation sequencing methodologies.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- Insights into tick-pathogen interactions - a single cell RNA sequencing approach of transcriptional changes during ehrlichial infection.bioRxiv : the preprint server for biology · 2026Article
- First transcriptome analysis of the winter tick (Dermacentor albipictus) reveals sex-specific expression patterns and potential targets for genetic control.G3 (Bethesda, Md.) · 2025Article
- Ticks' tricks: immunomodulatory effects of ixodid tick saliva at the cutaneous tick-host interface.Frontiers in immunology · 2025Review
- Serpins in the Spotlight: Novel Bioinformatic Insights IntoBiochemistry research international · 2025Article
- An Unusual Two-Domain Thyropin from Tick Saliva: NMR Solution Structure and Highly Selective Inhibition of Cysteine Cathepsins Modulated by Glycosaminoglycans.International journal of molecular sciences · 2024Article
- The immune factors involved in the rapid clearance of bacteria from the midgut of the tickFrontiers in cellular and infection microbiology · 2024Article
- Protease-bound structure of Ricistatin provides insights into the mechanism of action of tick salivary cystatins in the vertebrate host.Cellular and molecular life sciences : CMLS · 2023Article
- Rabbits as Animal Models for Anti-Tick Vaccine Development: A Global Scenario.Pathogens (Basel, Switzerland) · 2023Review
- More than Three Decades of Bm86: What We Know and Where to Go.Pathogens (Basel, Switzerland) · 2023Review
- Conformational transition of the Ixodes ricinus salivary serpin Iripin-4.Acta crystallographica. Section D, Structural biology · 2023Article
- Defensins as a promising class of tick antimicrobial peptides: a scoping review.Infectious diseases of poverty · 2022Article
- Review
- Transcriptomic analysis of the tick midgut and salivary gland responses upon repeated blood-feeding on a vertebrate host.Frontiers in cellular and infection microbiology · 2022Article
- Host Immune Responses to Salivary Components - A Critical Facet of Tick-Host Interactions.Frontiers in cellular and infection microbiology · 2022Review
- rDromaserpin: A Novel Anti-Hemostatic Serpin, from the Salivary Glands of the Hard TickToxins · 2021Article
- De novo assembled salivary gland transcriptome and expression pattern analyses for Rhipicephalus evertsi evertsi Neuman, 1897 male and female ticks.Scientific reports · 2021Article
- The sialotranscriptome of the gopher-tortoise tick, Amblyomma tuberculatum.Ticks and tick-borne diseases · 2021Article
- Transcriptomic Analysis of Salivary Glands ofFrontiers in physiology · 2021Article
- Tick Immune System: What Is Known, the Interconnections, the Gaps, and the Challenges.Frontiers in immunology · 2021Review
- Iripin-3, a New Salivary Protein Isolated FromFrontiers in immunology · 2021Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
Abstract
Tick salivary gland (SG) proteins possess powerful pharmacologic properties that facilitate tick feeding and pathogen transmission. For the first time, SG transcriptomes of Ixodes ricinus, an important disease vector for humans and animals, were analyzed using next-generation sequencing. SGs were collected from different tick life stages fed on various animal species, including cofeeding of nymphs and adults on the same host. Four cDNA samples were sequenced, discriminating tick SG transcriptomes of early- and late-feeding nymphs or adults. In total, 441,381,454 pyrosequencing reads and 67,703,183 Illumina reads were assembled into 272,220 contigs, of which 34,560 extensively annotated coding sequences are disclosed; 8686 coding sequences were submitted to GenBank. Overall, 13% of contigs were classified as secreted proteins that showed significant differences in the transcript representation among the 4 SG samples, including high numbers of sample-specific transcripts. Detailed phylogenetic reconstructions of two relatively abundant SG-secreted protein families demonstrated how this study improves our understanding of the molecular evolution of hematophagy in arthropods. Our data significantly increase the available genomic information for I. ricinus and form a solid basis for future tick genome/transcriptome assemblies and the functional analysis of effectors that mediate the feeding physiology and parasite-vector interaction of I. ricinus.
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