ArticleStem cells (Dayton, Ohio)2014
Akt suppression of TGFβ signaling contributes to the maintenance of vascular identity in embryonic stem cell-derived endothelial cells.
Article in Stem cells (Dayton, Ohio), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- cAMP/EPAC Signaling Enables ETV2 to Induce Endothelial Cells with High Angiogenesis Potential.Molecular therapy : the journal of the American Society of Gene Therapy · 2020Article
- SOX Transcription Factors in Endothelial Differentiation and Endothelial-Mesenchymal Transitions.Frontiers in cardiovascular medicine · 2019Article
- Conversion of human adipose-derived stem cells into functional and expandable endothelial-like cells for cell-based therapies.Stem cell research & therapy · 2018Article
- TGF-β Signaling in Control of Cardiovascular Function.Cold Spring Harbor perspectives in biology · 2018Review
- Inhibition of TGF-β Signaling in SHED Enhances Endothelial Differentiation.Journal of dental research · 2018Article
- Vascular endothelium plays a key role in directing pulmonary epithelial cell differentiation.The Journal of cell biology · 2017Article
- Article
- Sox17 drives functional engraftment of endothelium converted from non-vascular cells.Nature communications · 2017Article
- Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells.Scientific reports · 2016Article
- Combinatorial extracellular matrix microenvironments promote survival and phenotype of human induced pluripotent stem cell-derived endothelial cells in hypoxia.Acta biomaterialia · 2016Article
- Effects of cellular origin on differentiation of human induced pluripotent stem cell-derived endothelial cells.JCI insight · 2016Article
- Matrix Gla protein regulates differentiation of endothelial cells derived from mouse embryonic stem cells.Angiogenesis · 2016Article
- Characterizing human pluripotent-stem-cell-derived vascular cells for tissue engineering applications.Stem cells and development · 2015Article
- Effects of concentrated growth factor on proliferation, migration, and differentiation of human dental pulp stem cells in vitro.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
The ability to generate and maintain stable in vitro cultures of mouse endothelial cells (ECs) has great potential for genetic dissection of the numerous pathologies involving vascular dysfunction as well as therapeutic applications. However, previous efforts at achieving sustained cultures of primary stable murine vascular cells have fallen short, and the cellular requirements for EC maintenance in vitro remain undefined. In this study, we have generated vascular ECs from mouse embryonic stem (ES) cells and show that active Akt is essential to their survival and propagation as homogeneous monolayers in vitro. These cells harbor the phenotypical, biochemical, and functional characteristics of ECs and expand throughout long-term cultures, while maintaining their angiogenic capacity. Moreover, Akt-transduced embryonic ECs form functional perfused vessels in vivo that anastomose with host blood vessels. We provide evidence for a novel function of Akt in stabilizing EC identity, whereby the activated form of the protein protects mouse ES cell-derived ECs from TGFβ-mediated transdifferentiation by downregulating SMAD3. These findings identify a role for Akt in regulating the developmental potential of ES cell-derived ECs and demonstrate that active Akt maintains endothelial identity in embryonic ECs by interfering with active TGFβ-mediated processes that would ordinarily usher these cells to alternate fates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.