ArticleJournal of visualized experiments : JoVE2013
An in-vitro preparation of isolated enteric neurons and glia from the myenteric plexus of the adult mouse.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 65 citations in OpenAlex.
- Interplay of the ENS and Microbiota With Murine Gut Epithelium-Derived Organoids in Aging.Aging cell · 2026Article
- Review
- Dual roles of GDNF in enteric glial cell plasticity: direct transdifferentiation via the CaMKII/NeuroD1 pathway and cooperative regulation in a neural stem cell-inducing medium.Stem cell research & therapy · 2026Article
- Rodent Enteric Glia Culture.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Electroacupuncture at ST36 Alleviates Visceral Hypersensitivity by Suppressing Eosinophil-Induced TRPV1 Expression on Duodenum.Pain research & management · 2026Article
- Butyrate prevents chemotherapy-induced gastrointestinal toxicity and microbial dysbiosis.Scientific reports · 2025Article
- Enteric glial S100B controls rhythmic colonic functions by regulating excitability and specificity in gut motor neurocircuits.The Journal of physiology · 2025Article
- Butyrate Modulates Intestinal Microbiome and Epithelial Function to Attenuate Irinotecan-induced GI Toxicity.Research square · 2025Article
- Modulation of Ceramide-Induced Apoptosis in Enteric Neurons by Aryl Hydrocarbon Receptor Signaling: Unveiling a New Pathway beyond ER Stress.International journal of molecular sciences · 2024Article
- A novel method for culturing enteric neurons generates neurospheres containing functional myenteric neuronal subtypes.Journal of neuroscience methods · 2024Article
- Review
- Fecal supernatants from dogs with idiopathic epilepsy activate enteric neurons.Frontiers in neuroscience · 2024Article
- Extracellular matrix substrates differentially influence enteric glial cell homeostasis and immune reactivity.Frontiers in immunology · 2024Article
- Amyloid-β mediates intestinal dysfunction and enteric neurons loss in Alzheimer's disease transgenic mouse.Cellular and molecular life sciences : CMLS · 2023Article
- Biglycan regulated colorectal cancer progress by modulating enteric neuron-derived IL-10 and abundance ofiScience · 2023Article
- Aryl hydrocarbon receptor activation affects nitrergic neuronal survival and delays intestinal motility in mice.Toxicological sciences : an official journal of the Society of Toxicology · 2023Article
- Comparative transcriptomics reveals highly conserved regional programs between porcine and human colonic enteric nervous system.Communications biology · 2023Article
- Methods to Study the Myenteric Plexus of Rat Small Intestine.Cellular and molecular neurobiology · 2023Article
- Impact of Siponimod on Enteric and Central Nervous System Pathology in Late-Stage Experimental Autoimmune Encephalomyelitis.International journal of molecular sciences · 2022Article
- Chronic Morphine Induces IL-18 in Ileum Myenteric Plexus Neurons Through Mu-opioid Receptor Activation in Cholinergic and VIPergic Neurons.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The enteric nervous system is a vast network of neurons and glia running the length of the gastrointestinal tract that functionally controls gastrointestinal motility. A procedure for the isolation and culture of a mixed population of neurons and glia from the myenteric plexus is described. The primary cultures can be maintained for over 7 days, with connections developing among the neurons and glia. The longitudinal muscle strip with the attached myenteric plexus is stripped from the underlying circular muscle of the mouse ileum or colon and subjected to enzymatic digestion. In sterile conditions, the isolated neuronal and glia population are preserved within the pellet following centrifugation and plated on coverslips. Within 24-48 hr, neurite outgrowth occurs and neurons can be identified by pan-neuronal markers. After two days in culture, isolated neurons fire action potentials as observed by patch clamp studies. Furthermore, enteric glia can also be identified by GFAP staining. A network of neurons and glia in close apposition forms within 5-7 days. Enteric neurons can be individually and directly studied using methods such as immunohistochemistry, electrophysiology, calcium imaging, and single-cell PCR. Furthermore, this procedure can be performed in genetically modified animals. This methodology is simple to perform and inexpensive. Overall, this protocol exposes the components of the enteric nervous system in an easily manipulated manner so that we may better discover the functionality of the ENS in normal and disease states.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.