ArticleThe Journal of biological chemistry2013
Mechanism for recognition of an unusual mycobacterial glycolipid by the macrophage receptor mincle.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
62 citing papers in PubMed, 133 citations in OpenAlex.
- Rationally designed tunable amphiphilic glycopolymers for safe and effective vaccine adjuvanticity.RSC advances · 2026Article
- Synthetic Ligands of Myeloid C-Type Lectin Receptors.Chembiochem : a European journal of chemical biology · 2026Review
- Review
- Linkage-Editing of 6,6'-Diacyl Neotrehalose and Evaluation of Mincle Activation Ability.Chemistry, an Asian journal · 2026Article
- Phylogenetic and structural insights into the origin of C-type lectin Mincle in vertebrates.Immunogenetics · 2025Article
- Preformed mincle dimers stabilized by an interchain disulfide bond in the neck region.Glycobiology · 2024Article
- The Dectin-1 and Dectin-2 clusters: C-type lectin receptors with fundamental roles in immunity.EMBO reports · 2024Review
- Review
- Systematic Evaluation of Regiochemistry and Lipidation of Aryl Trehalose Mincle Agonists.International journal of molecular sciences · 2024Article
- Probing Isosteric Replacement for Immunoadjuvant Design: Bis-Aryl Triazole Trehalolipids are Mincle Agonists.ACS medicinal chemistry letters · 2024Article
- Secondary Sites of the C-type Lectin-Like Fold.Chemistry (Weinheim an der Bergstrasse, Germany) · 2024Review
- Pathogenicity and virulence ofVirulence · 2023Review
- PGL-III, a Rare Intermediate ofACS central science · 2023Article
- Thermophobic Trehalose Glycopolymers as Smart C-Type Lectin Receptor Vaccine Adjuvants.Advanced healthcare materials · 2023Article
- Synthesis and Biological Evaluation of Trehalose-based Bi-aryl Derivatives as C-type Lectin Ligands.Tetrahedron · 2023Article
- Characterisation of the bovine C-type lectin receptor Mincle and potential evidence for an endogenous ligand.Frontiers in immunology · 2023Article
- Versatile approach towards fully desymmetrized trehalose with a novel set of orthogonal protecting groups.Frontiers in chemistry · 2023Article
- Recognition of Tumor Nidogen-1 by Neutrophil C-Type Lectin Receptors.Biomedicines · 2022Article
- Water-soluble trehalose glycolipids show superior Mincle binding and signaling but impaired phagocytosis and IL-1β production.Frontiers in molecular biosciences · 2022Article
- Medicinal chemistry of the myeloid C-type lectin receptors Mincle, Langerin, and DC-SIGN.RSC medicinal chemistry · 2021Review
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Binding of the macrophage lectin mincle to trehalose dimycolate, a key glycolipid virulence factor on the surface of Mycobacterium tuberculosis and Mycobacterium bovis, initiates responses that can lead both to toxicity and to protection of these pathogens from destruction. Crystallographic structural analysis, site-directed mutagenesis, and binding studies with glycolipid mimics have been used to define an extended binding site in the C-type carbohydrate recognition domain (CRD) of bovine mincle that encompasses both the headgroup and a portion of the attached acyl chains. One glucose residue of the trehalose Glcα1-1Glcα headgroup is liganded to a Ca(2+) in a manner common to many C-type CRDs, whereas the second glucose residue is accommodated in a novel secondary binding site. The additional contacts in the secondary site lead to a 36-fold higher affinity for trehalose compared with glucose. An adjacent hydrophobic groove, not seen in other C-type CRDs, provides a docking site for one of the acyl chains attached to the trehalose, which can be targeted with small molecule analogs of trehalose dimycolate that bind with 52-fold higher affinity than trehalose. The data demonstrate how mincle bridges between the surfaces of the macrophage and the mycobacterium and suggest the possibility of disrupting this interaction. In addition, the results may provide a basis for design of adjuvants that mimic the ability of mycobacteria to stimulate a response to immunization that can be employed in vaccine development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.